Mouse C4 (Complement Component 4) ELISA Kit

CAT: 0855-ELK10632-01Size: 48 TestsDry Ice: NoHazardous: No
CAT#:0855-ELK10632-01Size:48 Tests
Selected
PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product image 1
1 / 1
Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse C4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse C4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse C4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse C4 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
C4BP-a; Complement Component 4 Binding Protein, Alpha
UniProt
P01029
Reactivity
Mouse
Field of Research
Signal transduction; Infection immunity; Hematology
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse C4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse C4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse C4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse C4 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
500 μg/mL
Sample Type
Serum, plasma and other biological fluids
Detection Range
7.82-500 μg/mL
Sensitivity
2.1 μg/mL

Related Products

CatalogName
ELK1653-01Human C4 (Complement Component 4) ELISA Kit
ELK11086-01Rat C4 (Complement Component 4) ELISA Kit
ELK11721-01Simian C4 (Complement Component 4) ELISA Kit
ELK10632MS-01Mouse C4 (Complement Component 4) Microsample ELISA Kit
ELK11086MS-01Rat C4 (Complement Component 4) Microsample ELISA Kit
ELK11123-01Mouse C4B (Complement C4-B) ELISA Kit
ELK1872-01Human C4B (Complement C4-B) ELISA Kit
ELK11123MS-01Mouse C4B (Complement C4-B) Microsample ELISA Kit
ELK1052-01Mouse C5a (Complement Component 5a) ELISA Kit
ELK10308-01Mouse C4a (Complement Component 4a) ELISA Kit

Alternative Products

CatalogName
MBS2883645-01Mouse Complement component C9 ELISA Kit
MBS100274-01Mouse Complement Component 6 ELISA Kit
MBS033527-01Mouse Complement Component 5 ELISA Kit
MBS040992-01Mouse Complement Component 3 ELISA Kit
MBS088894-01Mouse Complement Component 5a ELISA Kit
MBS014985-01Mouse Complement Component 3B ELISA Kit
MBS720540-01Mouse Complement Component 1R ELISA Kit
GTR10414725Mouse Complement Component 9 ELISA kit
MBS733375-01Mouse Complement Component 8b ELISA Kit
MBS724564-01Mouse Complement Component 4B ELISA Kit

Popular Products