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Mouse LEPR (Leptin Receptor) Microsample ELISA Kit

CAT: 0855-ELK10393MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK10393MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
CD295; LEPR; OBR; LR
UniProt
P48356
Reactivity
Mouse
Field of Research
Signal transduction; CD & Adhesion molecule; Metabolic pathway; Endocrinology
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse LEPR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse LEPR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse LEPR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse LEPR in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
200 ng/mL
Sample Type
Serum, plasma, tissue homogenates and other biological fluids.
Detection Range
3.13-200 ng/mL
Sensitivity
1.22 ng/mL

UniProtKB · P48356

Leptin receptor

LEPR_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P48356
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Lepr
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
LEP-R
EC number
—
Processing
Precursor
Secondary accessions
O35686, O54986, Q61215, Q64309, Q9QWG3, Q9QWV5
Protein keywords

Technical term

3D-structureReference proteome

Coding sequence diversity

Alternative splicing

Cellular component

Cell membraneMembraneSecreted

PTM

Disulfide bondGlycoproteinPhosphoprotein

Domain

Immunoglobulin domainRepeatSignalTransmembraneTransmembrane helix

Disease

Obesity

Molecular function

Receptor

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