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Human Leptin (LEP) Microsample Fast ELISA Kit

CAT: 0013-GTR18853691-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18853691-01Size:48 Tests
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Description
Human Leptin (LEP) Microsample Fast ELISA Kit
Product Name Alternative
LEPD, OBS, Obesity Homolog
UniProt
P41159
Reactivity
Human
Target
LEP
Field of Research
Metabolism Research
Assay Type
Sandwich
Assay Performance Time
3H
Sample Type
Serum, plasma, Tissue homogenate and Other biological samples; Sample volume:25μL
Sensitivity
9.38 pg/mL
Notes
For research use only.
Applications Notes
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human LEP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LEP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LEP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human LEP. You can calculate the concentration of Human LEP in the samples by comparing the OD of the samples to the standard curve.
Dynamic Range
15.63-1000pg/mL

UniProtKB · P41159

Leptin

LEP_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P41159
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
LEP
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
O15158, Q56A88
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Disease

Diabetes mellitusDisease variantObesity

PTM

Disulfide bond

Cellular component

Secreted

Domain

Signal

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