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Rat LEPR (Leptin Receptor) Microsample ELISA Kit

CAT: 0013-GTR18872915-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18872915-01Size:48 Tests
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Description
Rat LEPR (Leptin Receptor) Microsample ELISA Kit
Product Name Alternative
CD295; LEPR; OBR; LR
UniProt
Q62959
Reactivity
Rat
Field of Research
Metabolism Research
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates and other biological fluids.
Sensitivity
0.64 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat LEPR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat LEPR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat LEPR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat LEPR in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
1.57-100 ng/mL

UniProtKB · Q62959

Leptin receptor

LEPR_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
Q62959
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Lepr
Protein existence
1: Evidence at protein level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
LEP-R
EC number
—
Processing
Precursor
Secondary accessions
O35772, O35773, O54805, P70493, P70494, P70495, P97589, Q62960, Q63007, Q63385, Q63386, Q9ERI4
Protein keywords

Coding sequence diversity

Alternative splicing

Cellular component

Cell membraneMembraneSecreted

Disease

Disease variantObesity

PTM

Disulfide bondGlycoproteinPhosphoprotein

Molecular function

Receptor

Technical term

Reference proteome

Domain

RepeatSignalTransmembraneTransmembrane helix

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