EasyStep Rat T4 (Thyroxine) ELISA Kit

• Description:

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat T4 antigen, and the Rat T4 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat T4 to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Rat T4 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative:

  3,5,3',5'-Tetraiodothyronine; L-Thyroxine; Levothyroxine

• Gene Aliases:

  3,5,3',5'-Tetraiodothyronine; L-Thyroxine; Levothyroxine

• Reactivity:

  Rat

• Field of Research:

  Endocrinology, Hormone metabolism

• Assay Type:

  Competitive Inhibition

• Assay Principle:

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat T4 antigen, and the Rat T4 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells , then added a HRP-conjugated antibody specific to Rat T4 to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Rat T4 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  1.5 H

• Standard:

  600 ng/mL

• Sample Type:

  Serum, plasma

• Detection Range:

  9.38 - 600 ng/mL

• Sensitivity:

  7 ng/mL

• Species:

  Rat

• CAS Number:

  7732-18-5