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T4 (Thyroxine) ELISA Kit

CAT: 0855-ELK1204-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK1204-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with T4 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to T4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of T4 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
3,5,3',5'-Tetraiodothyronine; L-Thyroxine; Levothyroxine
Reactivity
General
Field of Research
Endocrinology; Hormone metabolism
Assay Type
Competitive Inhibition
Assay Principle
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with T4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to T4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of T4 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
2h
Standard
300 ng/mL
Sample Type
Serum, plasma and other biological fluids
Detection Range
4.69-300 ng/mL
Sensitivity
1.48 ng/mL

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