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Mouse Estradiol (E2) EasyStep ELISA Kit

CAT: 0013-GTR18859404-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18859404-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Mouse Estradiol (E2) EasyStep ELISA Kit
Product Name Alternative
17B-Estradiol; Oestradiol; Beta-Estradiol
Reactivity
Mouse
Target
E2
Field of Research
Pharmacology & Drug Discovery
Assay Type
Competitive
Assay Performance Time
1.5h
Sample Type
Serum
Sensitivity
25 pg/mL
Notes
For research use only.
Applications Notes
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse E2 antigen, and the Mouse E2 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells , then added a HRP-conjugated antibody specific to Mouse E2 to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Mouse E2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Tested Applications
ELISA
Dynamic Range
25-1600 pg/mL

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