REACH Cyprinid Vitellogenin ELISA (TECO®)

• Description:

  The REACH Cyprinid Vitellogenin ELISA Kit is a sensitive sandwich enzyme linked immunosorbent assay for the quantitative determination of vitellogenin in serum, WBH and mucus of cyprinids according to EC regulation Nr. 440/2008 (REACH) from July 10th 2015/ Document D039048/03

• Sample Volume:

  50 µL

• Detection Range:

  0.4-70 ng/mL

• Sensitivity:

  LLOQ 0.04 ng/mL LLD 0.025 ng/mL

• Components:

  Standards:       Standard Stock, lyophilized, 2 vials Controls: 3 Control levels, lyophilized, 2 vials each Inter-Assay Reference Standard Stock, lyophilized, 1 vial NSB-Strip:      1 breakable strip of 8 wells Sample preparationSerum: Store fresh serum samples immediately after collection at -20°C or lower until assayed. WBH: Store fresh WBH samples immediately after collection at -20°C or lower until assayed. Collect mucus as described in the TECO® Mucus Collection Set (TE1034) . Mucus-containing swabs can be stored several months at <-20°C Reference valuesSerum levels are in the range of µg/mL up to mg/mL WBH levels are in the range of mg/mL Mucus level are in the range of ng/mL

• Species:

  Fish Cyprinid Carp (Caprinus carpio) Goldfish (Carassius auratus) Zebrafish (Danio rerio) Fathead Minnow (Pimephales promelas)

• Incubation Time:

  4.0 Hours