Ultra Sensitive Cyprinid Vitellogenin ELISA (TECO®)

• Description:

  The Ultra Sensitive Cyprinid Vitellogenin ELISA Kit is a very sensitive sandwich enzyme linked immunosorbent assay for the quantitative determination of vitellogenin in samples obtained from cell culture and mucus of cyprinids.

• Sample Volume:

  50 µL

• Detection Range:

  0.025 -2.0 ng/mL

• Sensitivity:

  LLOQ < 0.025 ng/mL LLD 0.002 ng/mL

• Components:

  Standards: Standard Stock, lyophilized, 2 vials Controls: 2 Control levels, lyophilized, 2 vials each Sample preparationMucus: Collect mucus as described in the TECO® Mucus Collection Set (TE1034) . Mucus-containing swabs can be stored several months at <-20°C. Cell culture: Store fresh samples immediately after collection at <-20°C until assayed. Reference valuesMucus level are in the range of ng/mL

• Species:

  Fish Carp (Cyprinus carpio) Goldfish (Carassius gibelio auratus) Zebrafish (Danio rerio) Fathead minnow (Pimephales promelas) "Common bream/freshwater bream/ bronze bream/carp bream" (Abramis brama) Roach (Rutilus rutilus) Common rudd  (Scardinius erythrophthalmus) Chub (Squalius cephalus) Common nase  (Chondrostoma nasus) Bleak (Alburnus alburnus) Neon Tetra (Paracheirodon innesi) Gudgeon (Gobio gobio) Common dace (Leuciscus leuciscus) Stone loach (Barbatula barbatula) Common minnow (Phoxinus phoxinus)

• Incubation Time:

  Overnight 16-24 Hours / 4h±10 min