Salmonid Vitellogenin ELISA (TECO®)

• Description:

  The Salmonid Vitellogenin ELISA Kit is a sensitive sandwich enzyme linked immunosorbent assay for the quantitative determination of vitellogenin in serum, WBH and mucus of salmonids.

• Sample Volume:

  50 µL

• Detection Range:

  0.4-35 ng/mL

• Sensitivity:

  LLOQ < 0.4 ng/mL LLD < 0.1ng/mL

• Components:

  Standards: Standard Stock, lyophilized, 2 vials Controls: 2 Control levels, lyophilized, 2 vials each Sample preparationSerum: Store fresh serum samples immediately after collection at -20°C or lower until assayed. WBH: Store fresh WBH samples immediately after collection at -20°C or lower until assayed. Collect mucus as described in the TECO® Mucus Collection Set (TE1034) . Mucus-containing swabs can be stored several months at <-20°C Reference valuesSerum levels are in the range of µg/mL up to mg/mL WBH levels are in the range of mg/mL Mucus level are in the range of ng/mL

• Species:

  Fish Atlantic salmon (Salmo salar) Brown trout (Salmo trutta) Chum salmon (Oncorhynchus keta) Pink salmon/humpback salmon (Oncorhynchus gorbuscha) Rainbow trout (Oncorhynchus mykiss) Brook trout (Salvelinus fontinalis) "Common whitefish, European whitefish" (Coregonus lavaretus) Maraena whitefish (Coregonus maraena)

• Incubation Time:

  4.0 Hours