Recombinant Mouse TIMP-2/TIMP2 protein (His tag)

• Background:

  Mouse Metalloproteinase inhibitor 2 (TIMP-2), belongs to a family of proteins that regulate the activation and proteolytic activity of matrix metalloproteinases (MMPs) . There are four mammalian members of the family, TIMP-1, TIMP-2, TIMP-3, and TIMP-4. The TIMP-2 is detected in testis, retina, hippocampus and cerebral cortex. The function of TIMP 2 protein is to inhibit MMPs non covalently by the formation of binary complexes. Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor.And the interaction with MMP-14 facilitates the activation of pro-MMP-2.It has been shown that the binding of TIMP 2 to a3b1 integrin results in the inhibition of endothelial cell proliferation and angiogenesis.

• Abbreviation:

  TIMP-2; TIMP2

• Synonyms:

  CSC-21Ktissue inhibitor of metalloproteinase 2; TIMP metalloproteinase inhibitor 2; Tissue inhibitor of metalloproteinase 2.; metalloproteinase inhibitor 2

• UniProt:

  P25785

• Accession Number:

  P25785

• Expression System:

  E.coli

• Tag:

  N-His

• Sequence:

  Cys 27-Pro 220

• Endotoxin:

  < 10 EU/mg of the protein as determined by the LAL method.

• Purity:

  > 95% as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  21.2 kDa

• Observed Molecular Weight:

  25 kDa

• Species:

  Mouse