Recombinant Human TIMP2/TIMP-2 Protein

Recombinant Human TIMP2/TIMP-2 Protein

• Background: Tissue inhibitors of metalloproteinases (TIMP) family are natural inhibitors of the matrix metalloproteinases (MMPs), the zinc enzymes involved in extracellular matrix maintenance and remodeling. The TIMP family encompasses four members (TIMP1-4), and they inhibit most MMPs by forming non-covalent binary complex. TIMP2 is a 22 kDa non N-glycosylated protein expressed by a variety of cell types, and plays a unique role among TIMP family members owing to its functions to regulate cellular responses to growth factors. Findings establish an unexpected, MMP-independent mechanism for TIMP2 inhibition of endothelial cell proliferation in vitro and reveal an important component of the antiangiogenic effect of TIMP2 in vivo. TIMP-2 thus is critical to the maintenance of tissue homeostasis and is involved in the regulation of tumor microenvironment.
• Synonyms: CSC-21K, DDC8, Metalloproteinase Inhibitor 2, Tissue Inhibitor of Metalloproteinases 2
• CAS Number: 9000-83-3
• UniProt: P16035
• Accession Number: NP_003246.1
• Host: HEK293 Cells
• Origin Species: Human
• Tag: None
• Sequence: Cys 27-Pro 220
• Applications: Enzyme
• Field of Research: Signal Transduction;Cell biology;Cardiovascular;Cancer;
• Purity: > 96 % as determined by reducing SDS-PAGE.
• Bioactivity: Measured by its ability to inhibit human MMP-2 cleavage of a fluorogenic peptide substrate MCA-PLGL-DPA-AR-NH2(R&D Systems, Catalog # ES001). The IC50 value is < 4 nM.
• Reconstitution: Please refer to the printed manual for detailed information.
• Molecular Weight: 20 kDa
• Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.
• Storage Conditions: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.