Recombinant Mouse TIMP1/TIMP Protein

• Background:

  Mouse Tissue Inhibitor of Metalloproteinases 1 (TIMP-1) is a member of TIMP family. The homologous proteins of TIMPs regulate the activity of matrix metalloproteinases (MMPs), including inhibition of active MMPs, proMMP activation, cell growth promotion, matrix binding, inhibition of angiogenesis and the induction of apoptosis. Timp-1 complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. It also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. It is known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13, and MMP-16, without MMP-14.

• Abbreviation:

  TIMP1; TIMP

• Synonyms:

  CLGI; Collagenase Inhibitor; EPA; Erythroid-Potentiating Activity; Fibroblast collagenase Inhibitor; Metalloproteinase Inhibitor 1; TIMP; TIMP-1; TIMP1; Tissue Inhibitor of Metalloproteinases 1

• UniProt:

  P12032

• Accession Number:

  P12032

• Expression System:

  HEK293 Cells

• Tag:

  None

• Sequence:

  Cys25-Arg205

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  20.2 kDa

• Observed Molecular Weight:

  26 kDa

• Species:

  Mouse