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Caspase 9 Activity Detection Substrate for Flow Cytometry

CAT: 0763-E-CK-A489-01Size: 20 TestsDry Ice: NoHazardous: No
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CAT#:0763-E-CK-A489-01Size:20 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Detection Method
Fluorometric method; Flow cytometry
Assay Principle
The Caspase 9 Activity Detection Substrate for Flow Cytometry are a novel fluorogenic dye that couple the caspase 9 recognition sequence (LEHD) to a high affinity DNA dye, which have cell membrane permeability and can penetrate the plasma membrane into the cytoplasm. The substrate itself has no fluorescence and has a charge exclusion effect with DNA. During cell apoptosis, caspase-9 cleaves the substrate and releases high affinity DNA dyes, which bind to DNA to produce strong fluorescence, thereby detecting caspase-9 activity and visualizing the morphological changes of the cell nucleus during apoptosis.
Sample Type
Cell samples
Shipping Conditions
Ice bags
Storage Conditions
This product can be stored at -20°C for 1 year in the dark.
Detection Instrument
Flow Cytometer; Fluorescence microscope

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