Caspase 1 Activity Detection Substrate for Flow Cytometry

CAT:

763-E-CK-A481-01

Size:

100 Tests

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Detection Method: Fluorometric method;Flow cytometry
Assay Principle: The Caspase 1 Activity Detection Substrate for Flow Cytometry are a novel fluorogenic dye that couple the caspase 1 recognition sequence (YVAD) to a high affinity DNA dye, which have cell membrane permeability and can penetrate the plasma membrane into the cytoplasm. The substrate itself has no fluorescence and has a charge exclusion effect with DNA. During cell apoptosis, caspase-1 cleaves the substrate and releases high affinity DNA dyes, which bind to DNA to produce strong fluorescence, thereby detecting caspase-1 activity and visualizing the morphological changes of the cell nucleus during apoptosis.
Assay Performance Time: 30 min
Sample Type: Cell samples
Shipping Conditions: Ice bag
Storage Conditions: This product can be stored at -20°C for 1 year in the dark.

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