Recombinant Human Laminin421 E8 Protein

Laminins are large molecular weight glycoproteins constituted by the assembly of three disulfide-linked polypeptides, the α, β and γ chains. The human genome encodes 11 genetically distinct laminin chains. Structurally, laminin chains differ by the number, size and organization of a few constitutive domains, endowing the various members of the laminin family with common and unique important functions. In particular, laminins are indispensable building blocks for cellular networks physically bridging the intracellular and extracellular compartments and relaying signals critical for cellular behavior, and for extracellular polymers determining the architecture and the physiology of basement membranes. Laminins are glycoproteins with both common and specific functions. One common and most important function of laminins is to interact with receptors anchored in the plasma membrane of cells adjacent to basement membranes. In doing so laminins regulate multiple cellular activities and signaling pathways. Laminins colocalized with NP41 within nerve sheath, particularly perineurium, where laminin-421 is predominant. Binding assays with phage expressing NP41 confirmed binding to purified laminin-421, laminin-211, and laminin-α4. Some data highlight the novel role of α4-laminins in tumor cell migration and identify laminin-421 as a primary ligand for MCAM and a putative mediator of tumor invasion and metastasis.

Laminin421; LN421

Q16363-2 & P55268 & P11047

N-6His&N-Flag&N-HA

HEK293 Cells

<0.01 EU/μg as determined by LAL test.

Lyophilized

Reconstitute the lyophilized protein in sterile water at a concentration of greater than 100 μg/mL.

92.7 & 25.8 & 28.5kDa

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. Please minimize freeze-thaw cycles and use a manual defrost freezer.

Glu629-His1449 & Leu1573–Gln1798 & Asn1364–Pro1609

Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.