Recombinant Human Laminin511 E8 Protein

Laminins are large molecular weight glycoproteins constituted by the assembly of three disulfide-linked polypeptides, the α, β and γ chains. The human genome encodes 11 genetically distinct laminin chains. Structurally, laminin chains differ by the number, size and organization of a few constitutive domains, endowing the various members of the laminin family with common and unique important functions. In particular, laminins are indispensable building blocks for cellular networks physically bridging the intracellular and extracellular compartments and relaying signals critical for cellular behavior, and for extracellular polymers determining the architecture and the physiology of basement membranes. Laminins are glycoproteins with both common and specific functions. One common and most important function of laminins is to interact with receptors anchored in the plasma membrane of cells adjacent to basement membranes. In doing so laminins regulate multiple cellular activities and signaling pathways. There are more than 50 theoretically possible heterotrimeric associations between all the a, β and c chains. laminins 511 and 521, are the most ubiquitous isoforms in the adult organism.

Laminin 511; LN511

O15230 & P07942 & P11047

N-His & N-Flag & N-HA

HEK293 Cells

<0.01 EU/μg as determined by LAL test.

>95% as determined by SDS-PAGE.

Lyophilized

Reconstitute the lyophilized protein in sterile water at a concentration of greater than 100 μg/mL.

86.7 & 26.4 & 28.5 KDa

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. Please minimize freeze-thaw cycles and use a manual defrost freezer.

Ala2534-Ala3323 & Leu1561-Leu1786 & Asn1364-Pro1609

Lyophilized from 0.2 μm filtered solution of PBS, pH7.4.