MMP26 Fluorogenic Assay Kit

• Background:

  MMP26 (also known as matrilysin-2 or endometase) is a member of the large family of matrix metalloproteinases (MMP) responsible for the breakdown of extracellular matrix proteins such as collagen. Most MMPs are secreted as inactive pro-proteins and are activated when cleaved by extracellular proteinases. MMP26 cleaves collagen type IV, vitronectin, fibronectin, and fibrinogen, among others. It also cleaves MMP9 for activation. MMPs are involved in tissue remodeling and in the escape of metastatic cells from the primary tumor. They are candidate therapeutic targets in various diseases related to tissue remodeling including cancer.

• Description:

  The MMP26 (Matrix metalloproteinase 26) Fluorogenic Assay Kit is designed to measure MMP26 protease activity for screening and profiling applications, in a homogeneous assay with no time-consuming washing steps. The kit contains enough recombinant MMP26 enzyme, substrate, and assay buffer for 96 reactions.Need us to run inhibitor screens or profile your compounds against MMP26? Check out ourProtease Screening Services.

• UniProt:

  Q9NRE1

• Applications:

  Screen or profile small molecule inhibitors of MMP26

• Format:

  Catalog #NameAmountStorageRecombinant MMP26*1 µg-80°C799191 mM MMP Substrate10 µl-80°C799171x MMP Assay Buffer 125 ml-20°C79685Black 96-well plate1Room Temp*The concentration of the protein is lot-specific and will be indicated on the tube.

• Shipping Conditions:

  -80°C

• Storage Conditions:

  This assay kit will perform optimally for up to6 months from date of receipt when the materials are stored as directed.

• Notes:

  Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For further questions, please email[email protected]

• Contraindications:

  The final concentration of DMSO in the assay should not exceed 1%.Fluorescent compounds that have λex=328 nm and detection at λem=393 nm can interfere with the readouts.The presence of strong acids or bases, ionic detergents and high salt should be avoided.