Mouse anti Yeast GAL-4 DNA Binding Domain
CAT:
579-Z150M
Size:
100 µg
Price:
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- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No
Mouse anti Yeast GAL-4 DNA Binding Domain
- Background: Binding domain of GAL4 is contained in the first 74 amino acids and contains a zinc finger sequence motif. However, amino acids 1-147 have been shown to bind to DNA in vitro. While GAL4 (1-74) bind DNA specifically, it is with a lower affinity than the GAL4 (1-147). GAL4 (1-147) binds to a specific 17 base pair nucleotide sequence, but alone is insufficient to activate transcription. Either of the two acidic activating regions, activating region I (amino acids 148-238) or activating region II (amino acids 768-881), must be joined to activate transcription.
- Synonyms: Mouse Anti GAL-4 DNA Binding region, gal4, gal 4 yeast
- CAS Number: 9007-83-4
- Host: Mouse
- Species Reactivity: Yeast
- Isotype: IgG1
- Clone: 8C-1
- Conjugation: Unconjugated
- Type: Monoclonal Antibody
- Applications: WB, IP
- Field of Research: Molecular Biology
- Purification Method: Protein A/G Chromatography
- Assay Principle: Recognizes a 106 kDa protein by Western blot in yeast cell extracts corresponding to amino acids 1-147 of the GAL4 DNA binding region. Western blot at 1-10 µg/mL. Optimal concentration should be evaluated by serial dilutions.
- Stability: See expiration date on vial
- Concentration: See vial for Concentration
- Form: Provided as solution in phosphate buffered saline with 0,08% sodium azide
- Precautions: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.
- References & Citations: 1. GAL4 is regulated by a glucose-responsive functional domain. Stone, G. , Sadowski, I. EMBO J. 1993, 12, 1375-1385. 2. An amino-terminal fragment of GAL4 binds DNA as a dimer. Carey, M., et al. J. Mol. Biol. 1989, 209, 423-432. 3. The DNA binding domains of the yeast Gal4 and Human c-Jun transcription factors interact through the zinc-finger and bZIP motifs. Sollerbrant, K., et al. Nucleic Acids Res. 1995, 23, 588-594.
- Shipping Conditions: Ambient Temperature, freeze upon arrival
- Storage Conditions: Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles
- Functional Analysis: WB