Mouse anti Lactoferrin

Mouse anti Lactoferrin

• Background: Lactoferrin (LF) is an iron-binding protein with bactericidal and bacteriostatic activity, which is stored within the secondary granules of granulocytes. LF expression is restricted to the post-mitotic maturation compartment of the granulocytic lineage, starting from the myelocyte stage. Normal and malignant myeloblasts are LF negative. The 4C5 antibody permits the identification and enumeration of Human granulocytes using flow cytometry. Results must be interpreted by a certified professional before final interpretation. Analyses performed with this antibody should be paralleled by positive and negative controls. If unexpected results are obtained which cannot be attributed to differences in laboratory procedures, please contact us.
• UniProt: P02788
• Host: Mouse
• Species Reactivity: Human
• Isotype: IgG1
• Clone: 4C5
• Type: Primary Antibodies
• Applications: Flow Cytometry, IF
• Purification Method: Purified by Chromatography
• Assay Principle: GM-4111 can be applied in immunohistochemistry on frozen and paraffin embedded tissues. For flow cytometric applications the following protocol is advised._x000D_ Permeabilization and Staining Procedure - In combination with our Permeabilization Kit FIX,PERM® (Cat. NoGAS-002) intracellular Lactoferrin can be easily stained in cell suspensions. - For each sample to be analyzed add 50 µL of whole blood, bone marrow or mononuclear cell suspension in a 5mL tube - Add 100 µL of Reagent A (Fixation Medium, stored and used at room temperature) - Incubate for 15 minutes at room temperature - Add 5mL phosphate buffered saline and centrifµge cells for 5 minutes at 300 g - Remove supernatant and add to cell pellet 100 µL Reagent B (Permeabilization Medium) and 20 µL of the Lactoferrin monoclonal antibody conjµgate - Vortex at low speed for 1-2 seconds - Incubate for 15 minutes at room temperature - Wash cells with phosphate buffered saline as described above - Remove supernatant and resuspend cells in sheath fluid for immediate analysis or resuspend cells in 0.5 mL 1.0 % formaldehyde and store them at 2-8°C in the dark. Analyze fixed cells within 24 hours. Conventional Staining for Microscopic Evaluations LF-Antibody 4C5 can also be used to demonstrate lactoferrin molecules by conventional immunofluorescence or immunoenzyme staining techniques on cell smears, cytospin preparations or tissue sections. Acetone or paraformaldehyde are suitable fixatives for these purposes.
• Form: Purified by Chromatography, Storage buffer: PBS pH 7,2, 1% BSA, 0,05% NaN3
• Precautions: For professional users only. This reagent contains sodium azide. To avoid the development of hazardous conditions, reagents containing azide should be diluted in running water prior to be discarded. Similar to the work with other biological products, proper handling procedures are recommended.
• References & Citations: 1. Braylan, R. C., Orfao, A., Borowitz, M. J. , Davis, B. H. (2001) Cytometry 46, 23-7. _x000D_ 2. Catovsky, D., Matutes, E., Buccheri, V., Shetty, V., Hanslip, J., Yoshida, N. , Morilla, R. (1991) Ann Hematol 62, 16-21. _x000D_ 3. Cowland, J. B. , Borregaard, N. (1999) J Leukoc Biol 66, 989-95_x000D_ 4. Cramer, E., Pryzwansky, K. B., Villeval, J. L., Testa, U. , Breton-Gorius, J. (1985) Blood 65, 423-32. _x000D_ 5. Groeneveld, K., te Marvelde, J. G., van den Beemd, M. WHooijkaas, H. , van Dongen, J. J. (1996) Leukemia 10, 1383-9. _x000D_ 6. Gullberg, U., Andersson, E., Garwicz, D., Lindmark, A. , Olsson, I. (1997) Eur J Haematol 58, 137-53. _x000D_ 7. He, J. , Furmanski, P. (1995) Nature 373, 721-4_x000D_ 8. Knapp, W., Majdic, O. , Strobl, H. (1993) Recent Results Cancer Res 131, 31-40. _x000D_ 9. Konikova, E., Glasova, M., Kusenda, J. , Babusikova, O. (1998) Neoplasma 45, 282-91. _x000D_ 10. Oehler, L., Majdic, O., Pickl, W. F., Stockl, J., Riedl, E., Drach, J., Rappersberger, K., Geissler, K. , Knapp, W. (1998) J Exp Med 187, 1019-28. _x000D_ 11. Paietta, E. (2003) Best Pract Res Clin Haematol 16, 671-83. _x000D_ 12. Rado, T. A., Bollekens, J., St Laurent, G., Parker, L. , Benz, E. J., Jr. (1984) Blood 64, 1103-9. _x000D_ 13. Rado, T. A., Wei, X. P. , Benz, E. J., Jr. (1987) Blood 70, 989-93. _x000D_ 14. Srivastava, C. H., Rado, T. A., Bauerle, D. , Broxmeyer, H. E. (1991) J Immunol 146, 1014-9. _x000D_ 15. Strobl, H. , Knapp, W. (2004) J Biol Regul Homeost Agents 18, 335-9. _x000D_ 16. Teng, C. T., Gladwell, W., Beard, C., Walmer, D., Teng, C. S. , Brenner, R. (2002) Mol Hum Reprod 8, 58-67.
• Storage Conditions: Nordic-MUbio monoclonal antibody reagents contain optimal concentrations of affinity-purified antibody; For stability reasons this monoclonal antibody solution contains sodium azide. These reagents should be stored at 2-8°C (DO NOT FREEZE!) and protected from prolonged exposure to light. If a slight precipitation occurs upon storage, this should be removed by centrifugation; It will not affect the performance or the concentration of the product Stability of the reagent: Please refer to the expiry date printed onto the vial. The use of the reagent after the expiration date is not recommended.