Mouse anti Yeast GAL-4 Activation Domain

• Background:

  GAL4 is a transcriptional activator which activates genes necessary for galactose catabolism. The DNA binding domain of GAL4 is contained in the first 74 amino acids and contains a zinc finger sequence motif. However, amino acids 1-147 have been shown to bind to DNA in vitro. While GAL4 (1-74) bind DNA specifically, it is with a lower affinity than the GAL4 (1-147). GAL4 (1-147) binds to a specific 17 base pair nucleotide sequence, but alone is insufficient to activate transcription. Either of the two acidic activating regions, activating region I (amino acids 148-238) or activating region II (amino acids 768-881), must be joined to activate transcription.

• Host:

  Mouse

• Species Reactivity:

  Yeast

• Isotype:

  IgG1

• Clone:

  5C8-5

• Conjugation:

  Unconjugated

• Type:

  Monoclonal Antibody

• Source:

  Hybridoma produced by the fusion of splenocytes from mice immunized with full length GAL4 expressed in E. Coli and mouse myeloma cells.

• Applications:

  WB, IP

• Field of Research:

  Molecular Biology

• Purification Method:

  Protein A/G Chromatography

• Assay Principle:

  Antibody can be used for Western Blotting (5-10µg/mL). Recognizes GAL4 derivatives containing the C-terminal activation domain. Optimal concentration should be evaluated by serial dilutions.

• Stability:

  See expiration date on vial

• Concentration:

  See vial for Concentration

• Form:

  Provided as solution in phosphate buffered saline with 0,08% sodium azide

• Precautions:

  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

• References & Citations:

  1. GAL4 is regulated by a glucose-responsive functional domain. Stone, G. , Sadowski, I. EMBO J. 1993, 12, 1375-1385._x000D_ 2. An amino-terminal fragment of GAL4 binds DNA as a dimer. Carey, M., et al. J. Mol. Biol. 1989, 209, 423-432._x000D_ 3. The DNA binding domains of the yeast Gal4 and Human c-Jun transcription factors interact through the zinc-finger and bZIP motifs. Sollerbrant, K., et al. Nucleic Acids Res. 1995, 23, 588-594.

• Shipping Conditions:

  Ambient Temperature, freeze upon arrival

• Storage Conditions:

  Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles

• Functional Analysis:

  WB

• CAS Number:

  9007-83-4