Products for Research Use Only
RegisterLogin

General Block ELISA Blocking Buffer

CAT: 0013-GTR19334027Size: 100 mLDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0013-GTR19334027Size:100 mL
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
Contains a mammalian protein blocking agent suitable for most antibody capture ELISA formats and peptide/protein antigen-down ELISA formats. It provides a stable long-term environment for dried antigen or antibody and minimizes non-specific binding.
Concentration
1 X
Storage Conditions
2-8°C
Notes
For research use only.
Applications Notes
1. Coat antibody or antigen onto the ELISA plate using ICT’s Antibody Coating Buffer or Antigen Coating Buffer. 2. Incubate 8–24 hours at room temperature (RT) . 3. Aspirate the coating solution. 4. Wash each well twice with ICT’s ELISA Wash Buffer. 5. Block the uncoated regions of the ELISA plate by pipetting 300–400 μL of blocking buffer into each well. Always use an equal or greater volume of blocking buffer than was used for the coating buffer solution. 6. Incubate 8–24 hours at RT. For best blocking, incubate overnight at RT. 7. Aspirate the blocking buffer; do not wash. 8. Run the assay immediately, or dry the plate for long-term storage and seal in a foil storage bag with a desiccant pack. Store dried and packaged plates at 2-8°C.

Related Products

CatalogName
GTR19301427Phosph-Free Block ELISA Blocking Buffer
GTR19349292Alternative Block ELISA Blocking Buffer, Synthetic
GTR19301429Phosph-Free Block ELISA Blocking Buffer
GTR19301428Phosph-Free Block ELISA Blocking Buffer
GTR19349294Alternative Block ELISA Blocking Buffer, Synthetic
GTR19349293Alternative Block ELISA Blocking Buffer, Synthetic
GTR19306808Monster Block ELISA Blocking Buffer, Non-Mammalian
GTR19306810Monster Block ELISA Blocking Buffer, Non-Mammalian
GTR19306809Monster Block ELISA Blocking Buffer, Non-Mammalian
GTR19338057ELISA Blocking Buffer

Alternative Products

CatalogName
633General Block ELISA Blocking Buffer
6299Alternative Block ELISA Blocking Buffer
6295Monster Block ELISA Blocking Buffer
MBS258384-01Neptune Block ELISA Blocking Buffer
6263Phosph-Free Block ELISA Blocking Buffer
MBS258395-01Alternative Block ELISA Blocking Buffer, Synthetic
MBS258394-01Monster Block ELISA Blocking Buffer, Non-Mammalian
GWB-E120C6Blocking Buffer for ELISA
MBS3907318-01ELISA Blocking Buffer
TBS5013Universal Block Buffer

Popular Products