General Block ELISA Blocking Buffer

General Block ELISA Blocking Buffer

• Description: Contains a mammalian protein blocking agent suitable for most antibody capture ELISA formats and peptide/protein antigen-down ELISA formats. It provides a stable long-term environment for dried antigen or antibody and minimizes non-specific binding.
• CAS Number: 7732-18-5
• Target Antigen: Necrotic and apoptotic cells
• Assay Principle: If fluorescence-stained cells cannot be evaluated immediately after labeling with the fluorescence probes, add Fixative to the cells at a ratio of 1:10. For example, add 100 µL Fixative to 900 µL cells and incubate for at least 15 minutes at RT in the dark. Never add Fixative until the staining and final wash steps have been completed. Fixed cells may be stored on ice or at 4°C up to 24 hours.
• pH: pH: 7.4 at 1X
• Shipping Conditions: Blue Ice
• Storage Conditions: 4°C
• Shelf Life: 24 months from date of manufacture