Physalin F

Physalin F is a natural blocker of CaV2.3 (R-type) and CaV2.2 (N-type) voltage-gated calcium channels.It is a secosteroid with potent anti-inflammatory and immunomodulatory activities. Physalin F induces apoptosis of PBMC, decreasing the spontaneous proliferation and cytokine production caused by Human T-lymphotropic virus type 1 (HTLV-1) infectionPhysalin F is a secosteroid with potent anti-inflammatory and immunomodulatory activities. A concentration-dependent inhibition of spontaneous proliferation of PBMC from HAM/TSP subjects was observed in the presence of physalin F, as evaluated by (3) H-thymidine uptake. The IC50 for physalin F was 0.97 0.11 μM. Flow cytometry analysis using Cytometric Bead Array (CBA) showed that physalin F (10 μM) significantly reduced the levels of IL-2, IL-6, IL-10, TNF-α and IFN-γ, but not IL-17A, in supernatants of PBMC cultures. Next, apoptosis induction was addressed by using flow cytometry to evaluate annexin V expression. Treatment with physalin F (10 μM) increased the apoptotic population of PBMC in HAM/TSP subjects. Transmission electron microscopy analysis of PBMC showed that physalin F induced ultrastructural changes, such as pyknotic nuclei, damaged mitochondria, enhanced autophagic vacuole formation, and the presence of myelin-like figures. In conclusion, physalin F induces apoptosis of PBMC, decreasing the spontaneous proliferation and cytokine production caused by HTLV-1 infection.

57423-71-9

>98% (HPLC)

[H][C@@]12C (=O) [C@]34O[C@]11[C@@] (C) (OC (=O) [C@@]1 (O) CC[C@@]1 ([H]) [C@@]3 ([H]) C[C@H]3O[C@]33CC=CC (=O) [C@]13C) [C@H]1C[C@]2 (C) [C@@] ([H]) (CO4) C (=O) O1

C28H30O10?

526.5

Storage temperature: -20°C. Stability: ≥ 2 years