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Recombinant Mouse Kit ligand (Kitlg), partial (Active)

CAT: 0013-GTR19149251-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR19149251-01Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recombinant Mouse Kit ligand (Kitlg), partial (Active)
Product Name Alternative
Kit ligand; Hematopoietic growth factor KL; Mast cell growth factor; MGF; Steel factor; Stem cell factor; SCF; c-Kit ligand [Cleaved into: Soluble KIT ligand; sKITLG]
UniProt
P20826
Expression Region
26-189aa
Tag
C-terminal hFc1-tagged
Source
Mammalian cell
Origin
Mus musculus (Mouse)
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Purity
Greater than 95% as determined by SDS-PAGE.
Bioactivity
1Measured by its binding ability in a functional ELISA.Immobilized Mouse Kit at 2 μg/mL can bind Mouse Kitlg. The EC50 is 19.35-24.80 ng/mL. 2Recombinant Mouse Kitlg captured on Protein A Chip can bind Recombinant Mouse Kit with an affinity constant of 15.1 nM as detected by MetaSPR Assay.
Form
Lyophilized powder
Molecular Weight
47.2 kDa
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes
For research use only.
Applications Notes
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Preservative
Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4
AA Sequence
KEICGNPVTDNVKDITKLVANLPNDYMITLNYVAGMDVLPSHCWLRDMVIQLSLSLTTLLDKFSNISEGLSNYSIIDKLGKIVDDLVLCMEENAPKNIKESPKRPETRSFTPEEFFSIFNRSIDAFKDFMVASDTSDCVLSSTLGPEKDSRVSVTKPFMLPPVA

UniProtKB · P20826

Kit ligand

SCF_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P20826
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Kitlg
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
P97332, Q3TNJ7, Q62524, Q64222, Q921N5
Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

Coding sequence diversity

Alternative splicing

Biological process

Cell adhesion

Cellular component

Cell membraneCell projectionCytoplasmCytoskeletonMembraneSecreted

PTM

Disulfide bondGlycoprotein

Molecular function

Growth factor

Domain

SignalTransmembraneTransmembrane helix

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