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Recombinant Human CD40L Protein

CAT: 0013-GTR19124386-01Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR19124386-01Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recombinant Human CD40 Ligand is produced by our Mammalian expression system and the target gene encoding Met113-Leu261 is expressed.
Product Name Alternative
CD40 Ligand; CD40-L; T-Cell Antigen Gp39; TNF-Related Activation Protein; TRAP; Tumor Necrosis Factor Ligand Superfamily Member 5; CD154; CD40LG; CD40L; TNFSF5; TRAP
UniProt
P29965
Expression Region
Met113-Leu261
Conjugation
Unconjugated
Tag
Tag free
Origin
Human
Field of Research
Immunology & Inflammation
Endotoxin
< 0.01 EU/μg as determined by LAL test.
Purity
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (QC verified)
Bioactivity
ELISA: Immobilized Anti-Human CD40L mAb (Cat. No.:orb2993151) at 5 μg/mL (100 μL/well) can bind Human CD40L (Cat. No.:orb2994532) . The EC50 of Human CD40L (Cat. No.:orb2994532) is ≤10 ng/mL. (QC Verified) . BLI: Loaded Human CD40-mFc (Cat#orb2994039) on AMC Biosensor, can bind Human CD40L (Cat#orb2994532) with an affinity constant of 10.2 nM as determined in BLI assay. Loaded Human CD40-Fc (Cat#orb2994010) on Protein A Biosensor, can bind Human CD40L (Cat#orb2994532) with an affinity constant of 3.29 nM as determined in BLI assay. Loaded Anti-Human CD40L mAb-Fc (Cat#orb2993151) on Protein A Biosensor, can bind Human CD40L (Cat#orb2994532) with an affinity constant of 0.02 nM as determined in BLI assay.
Form
Lyophilized
Molecular Weight
Predicted: 16.2 KDa. Observed: 16-18 KDa, reducing conditions
Storage Conditions
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Notes
For research use only.
Preservative
0.2 μm filtered solution of 20mM PB, 200mM NaCl, 0.1mM EDTA, pH 7.0.

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