Hydrogen Peroxide Detection/Peroxidase Detection Kit

CAT: 0436-FLOH100-3Size: 500 TestsDry Ice: NoHazardous: No

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CAT#:0436-FLOH100-3Size:500 Tests

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Description

A rapid, no-wash assay to measure H2O2, based on the formation of a fluorescent product. The kit can measure H2O2 release from cells or enzyme coupled reactions. It can also measure peroxidase activity.

Label

ICT

Type

Cell Viability

Detection Method

Fluorescence plate reader

Assay Protocol

https://cdn.shopify.com/s/files/1/0512/5793/4009/files/Cell_Technology_Protocol_for_Fluoro_H2O2_FLOH100-3_Detection_Kit.pdf?v=1693512640

Components

Part # 3011: 5X Reaction Buffer: 20 ml buffer, pH 7.4 (2-8°C), Part # 4007: Detection reagent: One vial for 500 assays. (2-8°C), Part # 3012: Hydrogen Peroxide: 200µL of a stabilized 3% solution. (2-8°C), Part # 6004: Horseradish Peroxidase: 18.9 Units of enzyme (2-8°C)

Shipping Conditions

Ships overnight (domestic), International Priority Shipping

Storage Temperature

2-8°C

Cellular Imaging & Detection

Oxidative Stress

Target Description

The kit utilizes a non-fluorescent detection reagent to measure H2O2. H2O2 oxidizes the detection reagent to produce a fluorescent product, resorufin which is catalyzed by peroxidase in a homogeneous no wash assay system. The detection reagent can be utilized to measure H2O2 release from cells or enzyme coupled reactions.

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Hydrogen Peroxide Detection/Peroxidase Detection Kit

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