Fluorescent Hypochlorite Detection Kit

CAT: 0436-FLOCL100-2Size: 100 TestsDry Ice: NoHazardous: No
CAT#:0436-FLOCL100-2Size:100 Tests
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Two cell-permeable dyes hydroxyphenyl fluorescein (HPF) and aminophenyl fluorescein (APF) detect hypochlorite production in cells. Hypochlorite production is observed by increased fluorescence of APF loaded cells with no increase in HPF loaded cells.
Label
ICT
Type
Cell Viability
Detection Method
Flow Cytometer, Fluorescence plate reader, Fluorescence Microscope
Components
Part# 4012: HPF Dye, 1 Vial (2-8C), Part# 4011: APF Dye, 1 Vial (2-8C)
Shipping Conditions
Ships overnight (domestic), International Priority Shipping
Storage Temperature
2-8°C
Cellular Imaging & Detection
Oxidative Stress
Target Description
Hydroxyphenyl fluorescein (HPF) and Aminophenyl fluorescein (APF) are selective for the detection of highly reactive oxygen species (hROS). HPF has little reactivity towards other ROS such as: singlet oxygen (O), superoxide (O2– •), hydrogen peroxide (H2O2), nitric oxide (NO•), and alkyl peroxide (RO2•). HPF/APF are cell permeable and can be used in combination to detect hypo- chlorite (-OCl) production in cells. Hypochlorite can be detected by loading two samples, one with APF and the other with HPF. Hypochlorite production is visualized by increase in fluorescence of APF loaded cells and no increase in fluorescence in HPF loaded cells.

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