Endogenous Peroxidase Block

CAT: 0436-AR-6586-01Size: 15 mLDry Ice: NoHazardous: No
CAT#:0436-AR-6586-01Size:15 mL
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Endogenous peroxidase and pseudoperoxidase activity is found in many frozen, formalin-fixed paraffin-embedded tissue sections and cell smears. Peroxidase is most commonly found in red blood cells, myoglobin in muscle cells, cytochrome in granulocytes, and monocytes, liver and kidney. The presence of endogenous peroxidase activity gives false results when using peroxidase based Immunohistochemistry technique. For frozen tissue sections eg. Peripheral blood, bone marrow, fixation should be done with acetone/methanol.
Description
Endogenous peroxidase and pseudoperoxidase activity is found in many frozen, formalin-fixed paraffin-embedded tissue sections and cell smears.
Specifications
Ready to Use Blocking Solution
Certification
RUO
Other Statements
For research use only; not for use in diagnostic procedures. FOR IN VITRO LABORATORY USE ONLY
Label
ICT
Type
IHC Reagents
Applications
IHC, ICC
Assay Protocol
1. Deparaffinizate the tissue as described in your protocol. 2. For paraffin sections add few drops of endogenous peroxidase blocking solution for 10 minutes at room temperature. For frozen sections use Endogenous Peroxidase blocking solution (1:10 diluted in methanol) . Rinse slide with distilled water 3X 3. Proceed with the protocol.
Sample Type
Cell Specially Blood, Frozen Tissues, Necrotic Tissues, FFPE Sections
Shipping Conditions
Ships overnight (domestic), International Priority Shipping
Target Description
Endogenous peroxidase and pseudoperoxidase activity is found in many frozen, formalin-fixed paraffin-embedded tissue sections and cell smears. Peroxidase is most commonly found in red blood cells, myoglobin in muscle cells, cytochrome in granulocytes, and monocytes, liver and kidney. The presence of endogenous peroxidase activity gives false results when using peroxidase based Immunohistochemistry technique. For frozen tissue sections eg. Peripheral blood, bone marrow, fixation should be done with acetone/methanol.

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