Endogenous Peroxidase Block
CAT:
436-AR-6586-01
Size:
15 mL
For Laboratory Research Only. Not for Clinical or Personal Use.
Price:
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- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No
Endogenous Peroxidase Block
Background:
Endogenous peroxidase and pseudoperoxidase activity is found in many frozen, formalin-fixed paraffin-embedded tissue sections and cell smears. Peroxidase is most commonly found in red blood cells, myoglobin in muscle cells, cytochrome in granulocytes, and monocytes, liver and kidney. The presence of endogenous peroxidase activity gives false results when using peroxidase based Immunohistochemistry technique. For frozen tissue sections eg. Peripheral blood, bone marrow, fixation should be done with acetone/methanol.Description:
Endogenous peroxidase and pseudoperoxidase activity is found in many frozen, formalin-fixed paraffin-embedded tissue sections and cell smears.Specifications:
Ready to Use Blocking SolutionCertification:
RUOOther Statements:
For research use only; not for use in diagnostic procedures. FOR IN VITRO LABORATORY USE ONLYLabel:
ICTType:
IHC ReagentsApplications:
IHC, ICCAssay Protocol:
1. Deparaffinizate the tissue as described in your protocol. 2. For paraffin sections add few drops of endogenous peroxidase blocking solution for 10 minutes at room temperature. For frozen sections use Endogenous Peroxidase blocking solution (1:10 diluted in methanol) . Rinse slide with distilled water 3X 3. Proceed with the protocol.Sample Type:
Cell Specially Blood, Frozen Tissues, Necrotic Tissues, FFPE SectionsShipping Conditions:
Ships overnight (domestic), International Priority ShippingTarget Description:
Endogenous peroxidase and pseudoperoxidase activity is found in many frozen, formalin-fixed paraffin-embedded tissue sections and cell smears. Peroxidase is most commonly found in red blood cells, myoglobin in muscle cells, cytochrome in granulocytes, and monocytes, liver and kidney. The presence of endogenous peroxidase activity gives false results when using peroxidase based Immunohistochemistry technique. For frozen tissue sections eg. Peripheral blood, bone marrow, fixation should be done with acetone/methanol.