Streptavidin Conjugated with Peroxidase

• Description:

  Streptavidin is a 52-66 kDa (average 60 KDa) (subunit MW 14 kDa) biotin-binding protein isolated from Streptomyces avidini. Streptavidin is superior to avidin, because it does not contain carbohydrate like avidin and has no net charge at neutral pH. Streptavidin~biotin system is routinely used in Immunohistochemistry (IHC).

• Other Statements:

  For research use only; not for use in diagnostic procedures. FOR IN VITRO LABORATORY USE ONLY

• Label:

  ICT

• Type:

  IHC Reagents

• Applications:

  WB, IHC, ICC, ELISA

• Assay Protocol:

  This product can be diluted in our Immuno Automation buffer IBSC cat # AR-6561 or PBS. BSA 0.5-1.0% should be added in these solutions. This product can also be diluted in our Universal antibody dilution buffer (AR-6562) without adding any carrier protein.

• Dilution:

  1:200-1:1:1,000 (ICC) ; 1:500-1:5,000 (WB)

• Buffer:

  The conjugate (one mg of streptavidin conjugated with peroxidase) is supplied in one ml of 10 mM PO4, 200 mM NaCl, pH 7.5, BSA 10 mg/ml and 0.05% Proclin 300.

• Shipping Conditions:

  Ships overnight (domestic), International Priority Shipping

• Storage Temperature:

  2-8°C

• Target Description:

  Streptavidin is a 52-66 kDa (average 60 KDa) (subunit MW 14 kDa) biotin-binding protein isolated from Streptomyces avidini. Streptavidin is superior to avidin, because it does not contain carbohydrate like avidin and has no net charge at neutral pH. Streptavidin~biotin system is routinely used in Immunohistochemistry (IHC). Extinction Coefficient 1% A280=32.0. Purity: > 99% pure by HPLC; Eletrophoretically homogenous, single band. Protein content: > 95%; Activity 14.8 units/ mg, one unit bunds 1.0 microgram of d-biotin; Protease activity: 0.000018 units/mg Isoelectric point: 7.0 +/- determined by Isoelectric focusing. Peroxidase used for conjugation is 44 kDa glycoprotein isolated from horseradish roots. The Rz (Reinhetszahl), absorbance ratio of A403/A275 is ~3.0 One mg of Streptavidin is conjugated with peroxidase, in equimolar ratio. Unconjugated Streptavidin is removed.