Anti-mCherry Antibody

• Background:

  MCherry is a red fluorescent protein derived from DsRed which is isolated from disc corals and related to GFP (green fluorescent protein) (Shaner et al., 2004) . Fluorescent proteins are commonly used to tag proteins in order to localize their expression in both cultured cells and in vivo. Antibodies against fluorescent proteins such as mCherry are especially useful when amplification of the fluorescent signal is necessary or desired. Our chicken anti-mCherry antibody is a highly sensitive reagent for detection of mCherry by western blot, immunocytochemistry, and immunohistochemistry.

• Description:

  Our Anti-mCherry chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects mCherry, and is affinity purified. It is great for use in ICC, WB.

• Short Description:

  Our Anti-mCherry chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects n/a mCherry, and is affinity purified. It is great for use in ICC, WB.

• Other Statements:

  These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

• Gene Name:

  MCHERRY

• UniProt:

  X5DSL3

• Host:

  Chicken

• Immunogen:

  Purified recombinant mCherry (Uniprot X5DSL3) expressed in E.Coli

• Immunogen Type:

  Purified recombinant mCherry (Uniprot X5DSL3) expressed in E.Coli

• Target:

  MCherry

• Clonality:

  Polyclonal

• Isotype:

  IgY

• Conjugation:

  Unconjugated

• Applications:

  ICC, WB

• Field of Research:

  Anti-Tag Antibodies

• Assay Type:

  Primary Antibody

• Assay Protocol:

  Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification

• Concentration:

  0.2 mg/mL

• Format:

  Affinity purified

• Form:

  Affinity purified

• Buffer:

  Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS, " pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

• Molecular Weight:

  27 kDa for native monomer

• Precautions:

  These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

• Shipping Conditions:

  Ice Packs

• Storage Conditions:

  Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

• Shelf Life:

  12 months from receipt of product

• WB Dilution:

  1:500-1:1000

• Pre Treatment:

  Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification

• Target Description:

  mCherry is a red fluorescent protein derived from DsRed which is isolated from disc corals and related to GFP (green fluorescent protein) (Shaner et al., 2004). Fluorescent proteins are commonly used to tag proteins in order to localize their expression in both cultured cells and in vivo. Antibodies against fluorescent proteins such as mCherry are especially useful when amplification of the fluorescent signal is necessary or desired. Our chicken anti-mCherry antibody is a highly sensitive reagent for detection of mCherry by western blot, immunocytochemistry, and immunohistochemistry.

• ICC Dilution:

  1:100-1:500

• Quality Control:

  Each new lot of this antibody is tested by Western blot on an mCherry overexpression cell lysate.

• Brid:

  AB_2910557

• Uniprot Accession Number:

  X5DSL3

• Physical Properties:

  Liquid

• Isotype:

  IgY