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Anti-Doublecortin Antibody

CAT: 0588-DCXSize: 1000 µLDry Ice: NoHazardous: No
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CAT#:0588-DCXSize:1000 µL
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Background
Doublecortin is a microtubule-associated protein found in neuroblasts and immature neurons, especially those undergoing embryonic migrations. It was first identified as the X-chromosome-encoded gene product mutated in the human diseases Lissencephaly (in males) and Periventricular Heterotopia (in females), diseases caused by failure of neocortical neuroblast migration. The human protein itself is 360 amino acids (40,044 daltons) and contains doublecortin motifs near its N-terminus that are believed to mediate the microtubule binding.
Description
Two antipeptide antibodies were generated in chickens against sequences shared between the mouse (AAT58219.1), rat (NP_445831.3) and human (CAA06617.1) gene products. These antibodies were affinity-purified and then mixed in equal concentrations. These antibodies have been validated with human, mouse and rat tissues.
Synonyms
Neuronal migration protein doublecortin, Doublin, Lissencephalin-X, Lis-X
Other Statements
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
Gene Name
DCX DBCN LISX
UniProt
O15075
Host
Chicken
Species Reactivity
Human, Mouse, Rat
Reactivity
Human, Mouse, and Rat
Immunogen
Synthetic peptide
Immunogen Type
Human, Mouse, Rat
Target
Doublecortin
Clonality
Polyclonal
Isotype
IgY
Conjugation
Unconjugated
Applications
IHC, ICC
Field of Research
Cell Markers Neuronal Markers
Assay Type
Primary Antibody
Assay Protocol
Chickens were immunized with two synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the doublecortin gene product, but are shared between the human (CAA06617.1, NCBI) and mouse (AAT58219.1, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity purified using a peptide column, and the concentrations of the eluates adjusted to 200 µg/mL. Finally, equal volumes of these affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.
Concentration
200 µg/mL
Dilution
WB Brain : : Not Recommended
Format
Affinity-Purified IgY
Form
Affinity-Purified IgY
Buffer
Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS, " pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.
Molecular Weight
40 kDa
Precautions
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
Shipping Conditions
Ice Packs
Storage Conditions
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.
Shelf Life
12 months from receipt of product
IHC Dilution
1:1000-1:2000
WB Dilution
Not Recommended
Pre Treatment
Chickens were immunized with two synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the doublecortin gene product, but are shared between the human (CAA06617.1, NCBI) and mouse (AAT58219.1, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity purified using a peptide column, and the concentrations of the eluates adjusted to 200 µg/mL. Finally, equal volumes of these affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.
Target Description
Doublecortin is a microtubule-associated protein found in neuroblasts and immature neurons, especially those undergoing embryonic migrations. It was first identified as the X-chromosome-encoded gene product mutated in the human diseases Lissencephaly (in males) and Periventricular Heterotopia (in females), diseases caused by failure of neocortical neuroblast migration. The human protein itself is 360 amino acids (40,044 daltons) and contains doublecortin motifs near its N-terminus that are believed to mediate the microtubule binding.
ICC Dilution
1:1000-1:2000
Quality Control
This anti-peptide antibody mixture was analyzed by immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.
Brid
AB_2313540
Uniprot Accession Number
O15075
Physical Properties
Liquid
Isotype
IgY
Product Datasheet
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