Anti-V5 Antibody

• Background:

  The V5 tag is derived from a protein epitope found in the P and V proteins of paramyxovirus from the simian virus 5 family. The V5 tag is generally added as an epitope tag to proteins using all 14 amino acids (GKPIPNPLLGLDST), but it may also be used with a shorter 9-amino acid (IPNPLLGLD) sequence. Proteins tagged with the V5 epitope tag are detected with high sensitivity and specificity using our chicken anti-V5 antibody using various applications including western blot, immunocytochemistry, and immunohistochemistry.

• Description:

  Our Anti-V5 chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects V5, and is affinity purified. It is great for use in ICC, WB.

• Short Description:

  Our Anti-V5 chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects n/a V5, and is affinity purified. It is great for use in ICC, WB.

• Other Statements:

  These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

• Host:

  Chicken

• Immunogen:

  Synthetic peptide encoding 14 amino acid V5 epitope fused to KLH carrier protein

• Immunogen Type:

  Synthetic peptide encoding 14 amino acid V5 epitope fused to KLH carrier protein

• Target:

  V5

• Clonality:

  Polyclonal

• Isotype:

  IgY

• Conjugation:

  Unconjugated

• Applications:

  ICC, WB

• Field of Research:

  Anti-Tag Antibodies

• Assay Type:

  Primary Antibody

• Assay Protocol:

  Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification

• Concentration:

  0.2 mg/mL

• Format:

  Affinity purified

• Form:

  Affinity purified

• Buffer:

  Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS, " pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

• Precautions:

  These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

• Shipping Conditions:

  Ice Packs

• Storage Conditions:

  Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

• Shelf Life:

  12 months from receipt of product

• WB Dilution:

  1:500-1:1000

• Pre Treatment:

  Eggs from hens hyperimmunized with target were used to prepare an IgY fraction which was then subjected to antigen-specific affinity purification

• Target Description:

  The V5 tag is derived from a protein epitope found in the P and V proteins of paramyxovirus from the simian virus 5 family. The V5 tag is generally added as an epitope tag to proteins using all 14 amino acids (GKPIPNPLLGLDST), but it may also be used with a shorter 9-amino acid (IPNPLLGLD) sequence. Proteins tagged with the V5 epitope tag are detected with high sensitivity and specificity using our chicken anti-V5 antibody using various applications including western blot, immunocytochemistry, and immunohistochemistry.

• ICC Dilution:

  1:100-1:500

• Quality Control:

  Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used in Western blot on a V5 overexpression cell lysate.

• Brid:

  AB_2910559

• Physical Properties:

  Liquid

• Isotype:

  IgY