Anti-Tau Antibody

• Background:

  Human Tau protein (i.e., microtubule-associated protein tau isoform 1) is a 78,902 dalton protein (758 amino acids) expressed in cortical neurons of the CNS. In normal individuals, tau contains moderate degrees of phosphorylation on various serine and threonine residues scattered throughout the N-terminal half of this protein. However in various neurodegenerative diseases, such as Alzheimer's disease, Tau becomes hyperphosphorylated on these residues, leading to the pathological formation of intracellular "neurofibrillary tangles." Such tangles are hallmarks of Alzheimer's disease, as well as various other neurodegenerative diseases, collectively known as the "tauopathies."

• Description:

  Antipeptide antibodies were generated in chickens against two sequences shared between the mouse (P10637.3) and human (P10636.5) gene products. Neither of these sequences contain phosphorylated serine or threonine residues, so these Tau antibodies recognize both the euphosphorylated and hyperphosphorylated forms of Tau. Antibodies were affinity-purified from IgY fractions prepared from yolks collected from hyperimmunized hens. These antibodies have been validated with human, mouse and rat tissues.

• Synonyms:

  Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

• Other Statements:

  These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

• Gene Name:

  MAPT MAPTL MTBT1 TAU

• UniProt:

  P10636

• Host:

  Chicken

• Species Reactivity:

  Human, Mouse, Rat

• Reactivity:

  Human, Mouse, and Rat

• Immunogen:

  Synthetic peptides

• Immunogen Type:

  Human, Mouse, Rat

• Target:

  Tau

• Clonality:

  Polyclonal

• Isotype:

  IgY

• Conjugation:

  Unconjugated

• Applications:

  IHC, ICC

• Field of Research:

  Cell Markers Neuronal Markers Axonal Markers Neurodegenerative Disease Alzheimer's Disease

• Assay Type:

  Primary Antibody

• Assay Protocol:

  Chickens were immunized with two synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different and nonphosphorylated regions of the Tau gene product, but are shared between the human (NP_05819, NCBI) and mouse (P01637, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity purified using a peptide column, and the concentrations of the eluates adjusted to 200 µg/mL. Finally, equal volumes of both of these affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

• Concentration:

  200 µg/mL

• Format:

  Affinity-Purified IgY

• Form:

  Affinity-Purified IgY

• Buffer:

  Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS, " pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

• Molecular Weight:

  55-62 kDa

• Precautions:

  These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

• Shipping Conditions:

  Ice Packs

• Storage Conditions:

  Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

• Shelf Life:

  12 months from receipt of product

• IHC Dilution:

  1:5000-1:10000

• Pre Treatment:

  Chickens were immunized with two synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different and nonphosphorylated regions of the Tau gene product, but are shared between the human (NP_05819, NCBI) and mouse (P01637, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity purified using a peptide column, and the concentrations of the eluates adjusted to 200 µg/mL. Finally, equal volumes of both of these affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

• Target Description:

  Human Tau protein (i.e., microtubule-associated protein tau isoform 1) is a 78,902 dalton protein (758 amino acids) expressed in cortical neurons of the CNS. In normal individuals, tau contains moderate degrees of phosphorylation on various serine and threonine residues scattered throughout the N-terminal half of this protein. However in various neurodegenerative diseases, such as Alzheimer's disease, Tau becomes hyperphosphorylated on these residues, leading to the pathological formation of intracellular "neurofibrillary tangles." Such tangles are hallmarks of Alzheimer's disease, as well as various other neurodegenerative diseases, collectively known as the "tauopathies."

• ICC Dilution:

  1:5000-1:10000

• Quality Control:

  Both of these anti-peptide antibodies were analyzed by immunohisto-chemistry (at a dilution of 1:20000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

• Brid:

  AB_2313563

• Uniprot Accession Number:

  P10636

• Physical Properties:

  Liquid

• Isotype:

  IgY