Anti-UmuD | Protein UmuD
CAT:
451-AS21 4546
Size:
100 µL
Price:
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- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No
Anti-UmuD | Protein UmuD
- Background: The products of umuD , umuC , and recA genes (SOS genes) are required for mutagenesis induced by radiation or chemical agents. Transcription of these SOS genes is repressed by a repressor, LexA protein in uninduced cells. Exposure of cells to DNA-damaging agents activates RecA protein to promote proteolytic cleavage of LexA protein. Inactivation of LexA protein by the cleavage consequently derepresses the SOS genes, umuD, C and recA . UmuD protein is then auto-cleaved, which is promoted by RecA protein ssDNA in a ATP-dependent manner. The processed UmuD protein is the active form for mutagenesis and the UmuD-UmuC complex functions as an error-prone translesion DNA polymerase.
- CAS Number: 9007-83-4
- Host: Rabbit
- Reactivity: Escherichia coli
- Immunogen: Highly purified, full length, recombinant UmuD protein fromE.coli,UniProt:P0AG11
- Clonality: Polyclonal
- Applications: Western blot (WB)
- Dilution: 1 : 3000 (WB)
- Purity: Serum. Contains 0.05 % sodium azide.
- Format: Liquid
- Molecular Weight: 17 kDa (intact) 14 kDa (processed)
- References & Citations: Shinagawaet al. (1998). RecA protein-dependent cleavage of UmuD protein and SOS mutagenesis. Proc Natl Acad Sci U S A. 1988 Mar;85(6):1806-10. doi: 10.1073/pnas.85.6.1806. PMID: 3126496; PMCID: PMC279868.
- Storage Conditions: Store at -20°C and make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.