Anti-RuvA | Holliday junction ATP-dependent DNA helicase

• Background:

  RuvAprotein ofEscherichia colibinds specifically to the Holliday structure which is the intermediate of recombination at the late stage of homologous recombination and recombination repair, and forms a complex with RuvB motor protein, allowing the migration of Holliday junction using ATP hydrolysis energy and expands the heteroduplex region. In solution, it forms a tetramer and binds to the cross-like DNA of the Holliday junction from below and above, holding it in between

• Host:

  Rabbit

• Reactivity:

  Escherichia coli

• Immunogen:

  Purified, full length RuvA protein ofEscherichia coli,UniProt:P0A809

• Clonality:

  Polyclonal

• Applications:

  ELISA (ELISA), Western blot (WB)

• Dilution:

  1 : 3000 (WB)

• Purity:

  Serum. Contains 0.05 % sodium azide.

• Format:

  Liquid

• Molecular Weight:

  22 kDa

• References & Citations:

  Shinagawa& Iwasaki (1996). Processing the holliday junction in homologous recombination. Trends Biochem Sci. 1996 Mar;21(3):107-11. PMID: 8882584.Iwasakiet al. (1992) Escherichia coli RuvA and RuvB proteins specifically interact with Holliday junctions and promote branch migration. Genes Dev. 1992 Nov;6(11):2214-20. doi: 10.1101/gad.6.11.2214. PMID: 1427081.

• Storage Conditions:

  Store at 4°C for up to 6 months, for longer storage-20°C is recommended; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

• CAS Number:

  9007-83-4