Anti-Phosphothreonine

• Background:

  Phosphothreonine is a phosphoamino acid and phosphorylated ester of threonine.

• HS Code:

  38221900

• Host:

  Rabbit

• Reactivity:

  Antibody detects proteins phosphorylated on threonine residues. Does not cross-react with phosphotyrosine.5 % BSA is recommened to use for blocking as milk contains casein which is a phospho protein.!!AIR8!!Antibody detects proteins phosphorylated on threonine residues. Does not cross-react with phosphotyrosine.5 % BSA is recommened to use for blocking as milk contains casein which is a phospho protein.

• Not reactive in:

  No confirmed exceptions from predicted reactivity are currently known

• Immunogen:

  KLH-conjugated phosphothreonine

• Clonality:

  Polyclonal

• Applications:

  ELISA (ELISA), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC), Western blot (WB)

• Field of Research:

  Plant Signal Transduction

• Dilution:

  ELISA (1:2000), ICC/IF (1:60), IP (1:100), WB (1:500)

• Purity:

  Immunogen affinity purified in PBS, 50% glycerol, 0.01% sodium azide.

• Format:

  Liquid

• Precautions:

  2 µg/mL of this antibody is sufficient for detection of phosphorylation signal in western blot using mouse spleen extract treated with Vanadium.Use freshly extracted samples. Precipitate target protein to purify it and avoid cross-reactions.

• Additionnal Information:

  Affinity purified in PBS, pH 7.4 with 0.09 % sodium azide and 50 % glycerol at concentration 0.25 mg/ml. This antibody is recognizing proteins and peptides phosphorylated on threonine residues. There are no cross reactions with phosphotyrosine.

• References & Citations:

  Weise Havshøi et al. (2024) . The mechanism behind tenuazonic acid-mediated inhibition of plant plasma membrane H+-ATPase and plant growth. JBC 107167.

• Storage Conditions:

  Store at -20°C for one year in storage buffer: PBS, 50 % glycerol and 0,01 % sodium azide, Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

• CAS Number:

  9007-83-4