Anti-Phosphothreonine

Anti-Phosphothreonine

• Background: Phosphothreonineis a phosphoamino acid and phosphorylated ester of threonine.
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Antibody detects proteins phosphorylated on threonine residues. Does not cross-react with phosphotyrosine.5 % BSA is recommened to use for blocking as milk contains casein which is a phospho protein.!!AIR8!!Antibody detects proteins phosphorylated on threonine residues. Does not cross-react with phosphotyrosine.5 % BSA is recommened to use for blocking as milk contains casein which is a phospho protein.
• Not reactive in: No confirmed exceptions from predicted reactivity are currently known
• Immunogen: KLH-conjugated phosphothreonine
• Clonality: Polyclonal
• Applications: ELISA (ELISA), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC), Western blot (WB)
• Dilution: ELISA (1:2000), ICC/IF (1:60), IP (1:100), WB (1:500)
• Purity: Immunogen affinity purified in PBS, 50% glycerol, 0.01% sodium azide.
• Format: Liquid
• Precautions: 2 µg/mL of this antibody is sufficient for detection of phosphorylation signal in western blot using mouse spleen extract treated with Vanadium.Use freshly extracted samples. Precipitate target protein to purify it and avoid cross-reactions.
• References & Citations: Weise Havshøiet al. (2024). The mechanism behind tenuazonic acid-mediated inhibition of plant plasma membrane H+-ATPase and plant growth. JBC 107167.
• Storage Conditions: Store at -20°C for one year in storage buffer: PBS, 50 % glycerol and 0,01 % sodium azide, Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.