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RuvA (protein, positive control)

CAT: 0451-AS21 4543PSize: 20 µgDry Ice: NoHazardous: No
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CAT#:0451-AS21 4543PSize:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
RuvA protein of Escherichia coli binds specifically to the Holliday structure which is the intermediate of recombination at the late stage of homologous recombination and recombination repair and forms a complex with RuvB motor protein allowing the migration of Holliday junction using ATP hydrolysis energy and expands the heteroduplex region. In solution, it forms a tetramer and binds to the cross-like DNA of the Holliday junction from below and above holding it in between.
HS Code
38221900
UniProt
P0A809
Applications
Western blot (WB)
Field of Research
Bacteria
Purity
Contains 50% glycerol, 10 mM Tris-HCl (pH 7,5), 2 mM EDTA, 100 mM NaCl, 5 mM mercaptoethanol. Over 90 % pure by SDS-PAGE.
Format
Liquid
Molecular Weight
22 kDa (monomer)
Precautions
RuvA protein is full length, highly purified (over 90 %, SDS-PAGE). UniProt:P0A809
Additionnal Information
This product can be used in functional studies as Holliday junction specific binding protein, which promotes Holliday-junction branch migration in combination with RuvB protein
References & Citations
Han et al. (2006) . Direct observation of DNA rotation during branch migration of Holliday junction DNA by Escherichia coli RuvA-RuvB protein complex. Proc Natl Acad Sci U S A. 2006 Aug 1;103 (31) :11544-8. doi: 10.1073/pnas.0600753103. Epub 2006 Jul 24. PMID: 16864792; PMCID: PMC1544206.Iwasaki et al. (1992) Escherichia coli RuvA and RuvB proteins specifically interact with Holliday junctions and promote branch migration. Genes Dev. 1992 Nov;6 (11) :2214-20. doi: 10.1101/gad.6.11.2214. PMID: 1427081.
Storage Conditions
Store at 4°C or -20°C for a longer period of time; once make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Product Datasheet
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