Anti-GFP | Green Fluorescence Protein, clone 1A5 (rat monoclonal)

Anti-GFP | Green Fluorescence Protein, clone 1A5 (rat monoclonal)

• Background: GFP (Green fluorescent protein)was originally identified in photo organs on jellyfishAequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.
• CAS Number: 9007-83-4
• Host: Rat
• Reactivity: Native GFP, Recombinant GFP (E. coli), all variants of GFP, including EGFP
• Immunogen: Recombinant GFP protein fromAequorea victoria,UniProt:P42212
• Clonality: Monoclonal
• Applications: Chromatin immunoprecipitation (ChIP), ELISA (ELISA), Immunofluorescence (IF), Immunoprecipitation (IP), Western blot (WB)
• Dilution: 1 : 5000-1 : 25 000 (ELISA), 1 : 500 (IF), 1 : 2000-1 : 10 000 (WB)
• Purity: Immunoglobulin Protein A purified in PBS, 50 % glycerol, filter sterilized.
• Format: Liquid, 1 mg/mL.
• References & Citations: Maeharaet al. (2015). issue-specific expression of histone H3 variants diversified after species separation. Epigenetics Chromatin. 2015 Sep 17;8:35. doi: 10.1186/s13072-015-0027-3. (ChIP)Okazakiet al. (2012). Nuclear localization signal in a cancer-related transcriptional regulator protein NAC1. Carcinogenesis. 2012 Oct;33(10):1854-62.doi: 10.1093/carcin/bgs193. (Immunoprecipiation)
• Storage Conditions: Store at -20°C, Avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.