Anti-GFP | Green Fluorescence Protein (affinity purified)

Anti-GFP | Green Fluorescence Protein (affinity purified)

• Background: GFP (Green fluorescent protein)was originally identified in photo organs on jellyfishAequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Native GFP, Recombinant GFP (E,coli), all variants of GFP, including EGFP
• Immunogen: Highly purified native GFP protein derived fromAequorea victoria,UniProt:P42212
• Clonality: Polyclonal
• Applications: ELISA (ELISA), Immunofluorescence (IF), Immunoprecipitation (IP), Western blot (WB)
• Dilution: 1 : 5000-1 : 25 000 (ELISA), 1: 100 - 1 : 500 (IF), 1 : 2000-1 : 10 000 (WB)
• Purity: Immunogen affinity purified serum in PBS pH 7.4.
• Format: Liquid
• Precautions: Minimal cross-reactivity withE.coliproteins.Antibody reactivity to mVenus or EBFP2 has not yet been determined.
• References & Citations: Canalet al. (2024).Cytochrome c levels affect the TOR pathway to regulate growth and metabolism under energy-deficient conditions. New Phytol. 2024 Mar;241(5):2039-2058.Huet al. (2023) Spatiotemporal formation of the large vacuole regulated by the BIN2-VLG module is required for female gametophyte development in Arabidopsis [published online ahead of print, 2023 Jan 17]. Plant Cell. 2023;koad007. doi:10.1093/plcell/koad007.Bellinvia, Garcia-Gonzalez J, Cifrova P, et al. (2022) CRISPR-Cas9 Arabidopsis mutants of genes for ARPC1 and ARPC3 subunits of ARP2/3 complex reveal differential roles of complex subunits. Sci Rep. 2022;12(1):18205. Published 2022 Oct 28. doi:10.1038/s41598-022-22982-8.Kalachovaet al. (2022) DIACYLGLYCEROL KINASE 5 Participates in Flagellin-Induced Signaling in Arabidopsis. Plant Physiol. 2022 Jul 28:kiac354. doi: 10.1093/plphys/kiac354. Epub ahead of print. PMID: 35900211.Sunet al. (2021) The epigenetic factor FVE orchestrates cytoplasmic SGS3-DRB4-DCL4 activities to promote transgene silencing in Arabidopsis. Sci Adv. 2021 Aug 4;7(32):eabf3898. doi: 10.1126/sciadv.abf3898. PMID: 34348894; PMCID: PMC8336953.Stelateet al. (2021) Correlative Light-Environmental Scanning Electron Microscopy of Plasma Membrane Efflux Carriers of Plant Hormone Auxin. Biomolecules. 2021 Sep 26;11(10):1407. doi: 10.3390/biom11101407. PMID: 34680040; PMCID: PMC8533460.Wieczoreket al. (2020) Development of a New Tomato Torrado Virus-Based Vector Tagged with GFP for Monitoring Virus Movement in Plants. Viruses. 2020 Oct 20;12(10):1195. doi: 10.3390/v12101195. PMID: 33092281; PMCID: PMC7588970.Kulichet al. (2018). Deubiquitinase OTU5 affects Root Responses to Phosphate Starvation. Plant Physiol. 2018 Jan 4. pii: pp.01693.2017. doi: 10.1104/pp.17.01693.
• Storage Conditions: Store at -20°C. Avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.