Anti-GFP | Green Fluorescence Protein, peroxidase conjugated

Anti-GFP | Green Fluorescence Protein, peroxidase conjugated

• Background: GFP (Green fluorescent protein)was originally identified in photo organs on jellyfishAequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa. This antibody is directly conjugated to soyabean peroxidase.
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Native GFP, Recombinant GFP (E,coli), all variants of GFP
• Immunogen: highly purified native GFP protein derived fromAequorea victoria,UniProt:P42212
• Clonality: Polyclonal
• Applications: ELISA (ELISA), Immunogold (IG), Immunohistochemistry (IHC), Western blot (WB)
• Dilution: 1 : 5000-1 : 25 000 (ELISA), 1:50 (IG), 1: 100-1:250 (IHC), 1 : 2000-1 : 10 000 (WB)
• Purity: Immunogen affinity purified serum in 10 mM Tris pH 8. Contains 0.02 % sodium azide. Soybean persoxidase conjugated.
• Format: Liquid
• Precautions: Minimal cross-reactivity withE.coliproteins
• Storage Conditions: Store at 4°C or in small aliquotes at -20°C; avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.