Anti-GFP | Green Fluorescence Protein

Anti-GFP | Green Fluorescence Protein

• Background: GFP (Green fluorescent protein)was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Recombinant GFP overexpressed inE.colifromArabidopsis thaliana, Chlamydomonas reinhardii, Nicotiana tabacum
• Immunogen: RecombinantGFP protein derived fromAequorea victoria,UniProt:P42212
• Clonality: Polyclonal
• Applications: Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), Western blot (WB)
• Dilution: 1: 5000 - 1 : 10 000 (WB)
• Purity: Immunogen affinity purified serum in PBS pH 7.4.
• Format: Lyophilized
• Reconstitution: For reconstitution add 50 µl of sterile water
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.