Anti-mAB-M | Mouse anti-human Abeta protein (3-10) region, oligomer-specific (clone 2D10,F6)

Anti-mAB-M | Mouse anti-human Abeta protein (3-10) region, oligomer-specific (clone 2D10,F6)

• Background: Alzheimer's disease (AD) is the most prevalent neurodegenerative disease in the growing population of elderly people. A hallmark of AD is the accumulation of plaques in the brain of AD patients. The plaques predominantly consist of aggregates of amyloid-beta (Abeta), a peptide of 39-42 amino acids generated in vivo by specific, proteolytic cleavage of the amyloid precursor protein. Recent findings however suggest that smaller oligomeric forms of Abeta, formed in parallel to the amyloid plaques, excert the predominant tissue damaging effect.Specific identification of the oligomeric forms is as a consequence of great interest. Based on a recently publishedtechniquea highly oligomer-specific antibody (mAB-M), targeting Abeta oligomers while omitting reactivity towards the monomeric and fibrillar counterpart, has been developed.
• CAS Number: 9007-83-4
• Host: Mouse
• Reactivity: Human
• Not reactive in: No confirmed exceptions from predicted reactivity are currently known
• Immunogen: Synthetic peptide chosen from human Abeta protein (3-10) pregion, oligomer specific
• Clonality: Monoclonal
• Applications: Dot blot (Dot), ELISA (ELISA), Immunolocalization (IL)
• Dilution: 10 µg/mL (IL), 1-2 µg/mL (Dot), 2-4 µg/mL (ELISA capture)
• Purity: Immunogen affinity purified serum in PBS pH 7.4.
• Format: Lyophilized
• Reconstitution: For reconstitution add 50 µl of sterile water
• Molecular Weight: 4.5 kDa
• Precautions: Due to location of antigen used to elicit this antibody in 3-10 region, it should bind to full length APP.
• References & Citations: Meilandtet al. (2019). Characterization of the selective in vitro and in vivo binding properties of crenezumab to oligomeric A[beta]. Alzheimers Res Ther. 2019 Dec 1;11(1):97. doi: 10.1186/s13195-019-0553-5.Brännströmet al. (2014). A Generic Method for Design of Oligomer-Specific Antibodies. PLoS ONE. DOI: 10.1371/journal.pone.0090857.
• Storage Conditions: For short-term storage, please add sodium azide and srote at +4°C. For long-term storage, store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.