Anti-mAB-O | Mouse anti-human Abeta protein (3-10) region, oligomer-specific (clone 3E5,F8)

Anti-mAB-O | Mouse anti-human Abeta protein (3-10) region, oligomer-specific (clone 3E5,F8)

• Background: Alzheimer's disease (AD) is the most prevalent neurodegenerative disease in the growing population of elderly people. A hallmark of AD is the accumulation of plaques in the brain of AD patients. The plaques predominantly consist of aggregates of amyloid-beta (Abeta), a peptide of 39-42 amino acids generated in vivo by specific, proteolytic cleavage of the amyloid precursor protein. Recent findings however suggest that smaller oligomeric forms of Abeta, formed in parallel to the amyloid plaques, excert the predominant tissue damaging effect.Specific identification of the oligomeric forms is as a consequence of great interest. Based on a recently publishedtechniquea highly oligomer-specific antibody (mAB-O), targeting Abeta oligomers while omitting reactivity towards the monomeric and fibrillar counterpart, has been developed.
• CAS Number: 9007-83-4
• Host: Mouse
• Reactivity: Human
• Not reactive in: No confirmed exceptions from predicted reactivity are currently known
• Immunogen: Synthetic peptide chosen from human Abeta (1-42) protein.
• Clonality: Monoclonal
• Applications: Dot blot (Dot), ELISA (ELISA), Immunolocalization (IL)
• Dilution: 10 µg/mL (IL), 1-2 µg/mL (Dot), 1 µg/mL (ELISA capture)
• Purity: Immunogen affinity purified serum in PBS pH 7.4.
• Format: Lyophilized
• Reconstitution: For reconstitution add 50 µl of sterile water
• Molecular Weight: 4.5 kDa
• Precautions: Immunolocalization:human tissue was paraffin-embedded and sectioned. De-waxed and rehydrated in an ethanol gradient. Antigens were retrieved in sodium citrate buffer (pH 6) at 95°C for 1 h. The tissue sections were separately incubated for 1 h at RT with primary antibody and antibody binding was visualized with IgG Preoxidase Reagent Kit.This Monoclonal IgG1, kappa light chain, (clone number 3E5.F8) is specific for human Amyloid-Beta oligomers.
• References & Citations: Brännströmet al. (2014). A Generic Method for Design of Oligomer-Specific Antibodies. PLoS ONE. DOI: 10.1371/journal.pone.0090857.
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.