Anti-PsbA | D1 protein of PSII, N-terminal

Anti-PsbA | D1 protein of PSII, N-terminal

• Background: ThepsbAgene has been cloned from many species of plants, green algae, and cyanobacteria. The psbA gene is located in the chloroplast genome and encodes for the D1 protein, a core component of Photosystem II. PsbA/D1 is rapidly cycled under illumination in all oxygenic photobionts. Tracking PsbA pools using the Global PsbA antibody can show the functional content of Photosystem II in a wide range of samples.
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Arabidopsis thaliana, Hordeum vulgare, Chlamydomonas reinhardtii, Chlorella vulgaris, Synechococcussp. PCC7942, sp. PCC7002,Synechocystis6803 substrain PCC-M
• Not reactive in: No confirmed exceptions from predicted reactivity are currently known
• Immunogen: KLH-conjugated synthetic peptide derived from N-terminal of available plant, algal and cyanobacterial PsbA sequences, includingArabidopsis thalianaUniProt:A4QJR4, TAIR:AtCg00020,Oryza sativaP0C434,Populus albaQ14FH6,Physcomitrella patensQ6YXN7,Chlamydomonas reinhardtiiP07753,Synechocystissp.P14660and many others
• Clonality: Polyclonal
• Applications: Western blot (WB)
• Dilution: 1 : 1000-1 : 10 000 (WB)
• Purity: Serum
• Format: Lyophilized
• Reconstitution: For reconstitution add 50 µl of sterile water
• Molecular Weight: 38 | 28-30 kDa
• Precautions: This antibody will detect the phosphorylated form of D1 as an alternate band to the main band on a high resolution gel
• References & Citations: Nagyet al. (2023). Photoautotrophic and sustained H2 production by the pgr5 mutant of Chlamydomonas reinhardtii in simulated daily light conditions. International Journal of Hydrogen Energy Volume 53, 31 January 2024, Pages 760-769.Vidal-Meireles, et al. (2023)The lifetime of the oxygen-evolving complex subunit PSBO depends on light intensity and carbon availability in Chlamydomonas. Plant Cell Environ. 2023;46(2):422-439. doi:10.1111/pce.14487.Neusiuset al. (2022) Lysine acetylation regulates moonlighting activity of the E2 subunit of the chloroplast pyruvate dehydrogenase complex in Chlamydomonas. Plant J. 2022 Sep;111(6):1780-1800. doi: 10.1111/tpj.15924. Epub 2022 Aug 8. PMID: 35899410.Chenet al. (2021)Degradation of the photosystem II core complex is independent of chlorophyll degradation mediated by Stay-Green Mg2+ dechelatase in Arabidopsis,Plant Science,Volume 307,2021,110902,ISSN 0168-9452,https://doi.org/10.1016/j.plantsci.2021.110902.Fukuraet al. (2021) Enrichment of chlorophyll catabolic enzymes in grana margins and their cooperation in catabolic reactions. J Plant Physiol. 2021 Nov;266:153535. doi: 10.1016/j.jplph.2021.153535. Epub 2021 Sep 25. PMID: 34607178.Terentyev(2020: The Main Structural and Functional Characteristics of Photosystem-II-Enriched Membranes Isolated From Wild Type and cia3 Mutant Chlamydomonas reinhardtii. Life (Basel). 2020 May 14;10(5):E63. doi: 10.3390/life10050063..Góreckaet al. (2019). Photosystem II 22kDa protein level a prerequisite for excess light-inducible memory, cross-tolerance to UV-C, and regulation of electrical signalling. Plant Cell Environ. 2019 Nov 23. doi: 10.1111/pce.13686.Liuet al. (2018). Effects of PSII Manganese-Stabilizing Protein Succinylation on Photosynthesis in the Model Cyanobacterium Synechococcus sp. PCC 7002. Plant Cell Physiol. 2018 Jul 1;59(7):1466-1482. doi: 10.1093/pcp/pcy080.Georget al. (2017). Acclimation of Oxygenic Photosynthesis to Iron Starvation Is Controlled by the sRNA IsaR1. Curr Biol. 2017 May 22;27(10):1425-1436.e7. doi: 10.1016/j.cub.2017.04.010.Perales-Velaet al. (2016). Streptomycin affects the growth and photochemical activity of the alga Chlorella vulgaris. Ecotoxicol Environ Saf. 2016 Oct;132:311-7. doi: 10.1016/j.ecoenv.2016.06.019. Epub 2016 Jun 23.Malnoëet al. (2014). Thylakoid FtsH Protease Contributes to Photosystem II and Cytochrome b6f Remodeling in Chlamydomonas reinhardtii under Stress Conditions. Plant Cell, Jan 21.SookSeok et al. (2013). AtFKBP16-1, a chloroplast lumenal immunophilin, mediates response to photosynthetic stress by regulating PsaL stability. Physiologia Plantarum, DOI: 10.1111/ppl.12116.
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.