Anti-PsbA | D1 protein of PSII, DE-loop

Anti-PsbA | D1 protein of PSII, DE-loop

• Background: ThepsbAgene has been cloned from many species of plants, green algae, and cyanobacteria. The psbA gene is located in the chloroplast genome and encodes for the D1 protein, a core component of Photosystem II. PsbA/D1 is rapidly cycled under illumination in all oxygenic photobionts. Tracking PsbA pools using the Global PsbA antibody can show the functional content of Photosystem II in a wide range of samples. Alternative names: 32 kDa thylakoid membrane protein, photosystem II protein D1
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Arabidopsis thaliana,Chlamydomonas reinhardii,Hordeum vulgare,Medicago truncatula, Nannochloropsis oceanica strain IMET1,Neochloris oleoabundansUTEX 1185 (chlorophyta), Nicotiana tabacum, Physcomitrium patens, Pisum sativum, Silene vulgaris, Sinapsis alba, Spinacia oleracea,Synechococcussp. PCC 7942,Synechocystissp. PCC6803,Triticumsp.
• Not reactive in: No confirmed exceptions from predicted reactivity are currently known
• Immunogen: KLH-conjugated synthetic peptide, amino acids 234-242 ofArabidopsis thalianaD1 protein UniProt:P83755, TAIR:AtCg00020
• Clonality: Polyclonal
• Applications: Blue Native PAGE (BN-PAGE), Western blot (WB)
• Dilution: 1 : 10 000, thylakoid fraction (WB)
• Purity: Serum
• Format: Lyophilized
• Reconstitution: For reconstitution add 50 µl of sterile water
• Molecular Weight: 38 | 28-30 kDa
• Precautions: Antibody is recognizing a 23 kDa fragment in spinach and Arabidopsis thylakoidsfor usage on total cell extracts the dilution needs to be determined experimentally
• References & Citations: Calderonet al. (2023) Rubredoxin 1 promotes the proper folding of D1 and is not required for heme b559 assembly in Chlamydomonas photosystem II, Journal of Biological Chemistry, 2023, 102968, ISSN 0021-9258.GaoY et al. (2022) Chloroplast translational regulation uncovers nonessential photosynthesis genes as key players in plant cold acclimation. Plant Cell. 2022 Apr 26;34(5):2056-2079. doi: 10.1093/plcell/koac056. PMID: 35171295; PMCID: PMC9048916.Ermakovaet al. (2022) Enhanced abundance and activity of the chloroplast ATP synthase in rice through the overexpression of the AtpD subunit. J Exp Bot. 2022 Jul 29:erac320. doi: 10.1093/jxb/erac320. Epub ahead of print. PMID: 35904136.Luet al. (2021). Role of an ancient light-harvesting protein of PSI in light absorption and photoprotection. Nat Commun. 2021 Jan 29;12(1):679. doi: 10.1038/s41467-021-20967-1. PMID: 33514722; PMCID: PMC7846763. (blue-native PAGE)Mazuret al. (2021) The SnRK2.10 kinase mitigates the adverse effects of salinity by protecting photosynthetic machinery. Plant Physiol. 2021 Dec 4;187(4):2785-2802. doi: 10.1093/plphys/kiab438. PMID: 34632500; PMCID: PMC8644180.Rantalaet al. (2020). PGR5 and NDH-1 systems do not function as protective electron acceptors but mitigate the consequences of PSI inhibition. Biochim Biophys Acta Bioenerg. 2020 Jan 11;1861(3):148154. doi: 10.1016/j.bbabio.2020.148154.Griecoet al. (2020). Adjustment of photosynthetic activity to drought and fluctuating light in wheat. Plant Cell Environ. 2020 Mar 16. doi: 10.1111/pce.13756.Rantalaand Tikkanen et al. (2018). Phosphorylation?induced lateral rearrangements of thylakoid protein complexes upon light acclimation. Plant Direct Vol. 2, Issue 2.Lianget al. (2018). Thylakoid-Bound Polysomes and a Dynamin-Related Protein, FZL, Mediate Critical Stages of the Linear Chloroplast Biogenesis Program in Greening Arabidopsis Cotyledons. Plant Cell. 2018 Jul;30(7):1476-1495. doi: 10.1105/tpc.17.00972. Epub 2018 Jun 7.Rantalaand Tikkanen et al. (2018). Phosphorylation‐induced lateral rearrangements of thylakoid protein complexes upon light acclimation. Plant Direct Vol. 2, Issue 2.Wuet al. (2018). Control of Retrograde Signaling by Rapid Turnover of GENOMES UNCOUPLED 1. Plant Physiol. 2018 Jan 24. pii: pp.00009.2018. doi: 10.1104/pp.18.00009.Giovanardiet al. (2017). Higher packing of thylakoid complexes ensures a preserved Photosystem II activity in mixotrophic Neochloris oleoabundans. Algal Research, Volume 25, July 2017, Pages 322-332.Kaleet al. (2017). Amino acid oxidation of the D1 and D2 proteins by oxygen radicals during photoinhibition of Photosystem II. Proc Natl Acad Sci U S A. 2017 Mar 14;114(11):2988-2993. doi: 10.1073/pnas.1618922114.Mazuret al. (2016). Overlapping toxic effect of long term thallium exposure on white mustard (Sinapis alba L.) photosynthetic activity. BMC Plant Biol. 2016 Sep 2;16(1):191. doi: 10.1186/s12870-016-0883-4.Kowalewskaet al. (2016). Three-dimensional visualization of the internal plastid membrane network during runner bean chloroplast biogenesis. Dynamic model of the tubular-lamellar transformation. The Plant Cell March 21, 2016 tpc.01053.2015.Fristedtet al. (2015). The thylakoid membrane protein CGL160 supports CF1CF0 ATP synthase accumulation in Arabidopsis thaliana. PLoS One. 2015 Apr 2;10(4):e0121658. doi: 10.1371/journal.pone.0121658.Karlssonet al. (2015). The Arabidopsis thylakoid transporter PHT4;1 influences phosphate availability for ATP synthesis and plant growth. Plant J. 2015 Aug 8. doi: 10.1111/tpj.12962.Malnoëet al. (2014). Thylakoid FtsH Protease Contributes to Photosystem II and Cytochrome b6f Remodeling in Chlamydomonas reinhardtii under Stress Conditions. Plant Cell, Jan 21.Sobrino-Plataet al. (2014). Glutathione is a key antioxidant metabolite to cope with mercury and cadmium stress. Plant Soil, DOI 10.1007/s11104-013-2006-4.Blocket al. (2013). Functional Modeling Identifies Paralogous Solanesyl Diphosphate Synthases that Assemble the Side Chain of Plastoquinone-9 in Plastids. J Biol Chem. Aug 2.Speteaet al. (1999). GTP bound to chloroplast thylakoid membranes is required for light-induced, multienzyme degradation of the photosystem II D1 protein. PNAS 96: 6547-6552.
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.