Anti-PsbA | D1 protein of PSII, C-terminal, HRP-conjugated (40 µg)

Anti-PsbA | D1 protein of PSII, C-terminal, HRP-conjugated (40 µg)

• Background: The psbA gene has been cloned from many species of plants, green algae, and cyanobacteria. The psbA gene is located in the chloroplast genome and encodes for the D1 protein, a core component of Photosystem II. PsbA/D1 is rapidly cycled under illumination in all oxygenic photobionts. Tracking PsbA pools using the Global PsbA antibody can show the functional content of Photosystem II in a wide range of samples. Alternative names: 32 kDa thylakoid membrane protein, photosystem II protein D1.
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Anabaena7120, Arabidopsis thaliana, Artemisia annua, Arundo sp., Chlamydomonas reinhardtii, Colobanthus quitensis Kunt Bartl, Coscinodiscus wailesii, Craterostigma sp., Ditylum brightwellii, Glycine max, Hordeum vulgare, Lindernia sp., Miscanthus x giganteus, Marchantia polymorpha (liverwort), Nicotiana benthamiana, Panicum miliaceum, Panax ginseng, Panicum maximum, Pinus strobus, Physcomitrium patens, Synechococcus sp. PCC7942, Paulinella chromatophora (amoeba), Prochlorococcus sp. (surface and deep water ecotype), Spirodela polyrhiza, Symbiodinium sp., Zea mays
• Not reactive in: No confirmed exceptions from predicted reactivity are currently known
• Immunogen: KLH-conjugated synthetic peptide derived from available plant, algal and cyanobacterial PsbA sequences, includingArabidopsis thalianaUniProt:A4QJR4, TAIR:AtCg00020,Oryza sativaP0C434,Populus albaQ14FH6,Physcomitrella patensQ6YXN7,Chlamydomonas reinhardtiiP07753,Synechocystissp.P14660and many others
• Clonality: Polyclonal
• Applications: Western blot (WB)
• Dilution: 1 : 15 000 (WB)
• Purity: Immunogen affinity purified serum in PBS pH 7.4, conjugated to HRP.
• Format: Liquid
• Molecular Weight: 38 | 28-30 kDa
• Precautions: The antibody is appropriate for detecting both, 24 kDa or the 10 kDa C-terminal fragments, whichever is generated under given treatment conditions. In our analysis we have seen both, ca. 24 kDa and ca. 10 kDa fragments from different samples, depending on treatments and isolation procedures.Rabbit anti-PsbA antibody can detect more than one band of PsbA protein, e.g. precursor and mature protein as compare to the hen anti-PsbA antibodies AS01 016.This antibody will detect the phosphorylated form of D1 as an alternate band to the main band on a high resolution gel.The antibody will bind to cross-linked proteins: D1/D2, D1/cyt b559, D1/CP43.
• References & Citations: Thurotteet al. (2020). DnaK3 Is Involved in Biogenesis and/or Maintenance of Thylakoid Membrane Protein Complexes in the Cyanobacterium Synechocystis Sp. PCC 6803. Life (Basel). 2020 Apr 30;10(5):E55. doi: 10.3390/life10050055.
• Storage Conditions: Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.