Anti-PsbA | D1 protein of PSII, C-terminal (chicken)

Anti-PsbA | D1 protein of PSII, C-terminal (chicken)

• Background: ThePsbA (D1)protein of Photosystem II is rapidly cycled under illumination in all oxygenic photobionts. Disruption of PsbA cycling or losses of PsbA pools are central to photoinhibition of photosynthesis in cyanobacteria, algae and plants under a wide range of conditions including excess light, low temperature and UV exposure. Tracking PsbA pools using the Global PsbA antibody can show the functional content of Photosystem II in a wide range of samples.
• CAS Number: 9007-83-4
• Host: Chicken
• Reactivity: Alaria esculenta, Amphidinium carterae, Anabaenasp., Arabidopsis thaliana, Brachypodium sylvaticum, Chlamydomonas reinhardtii, Chlamydomonas raudensis(both Antarctic and mesophilic strains),Cyanophorasp. ,Cyanothece sp.ATCC 51142, Cynara cardunculus, Gonyaulax polyedra, Fucus vesiculosus, Horderum vulgare, Lobaria pulmonaria, Petuniasp.,Pinus sylvestris, Spartina alterniflora, Solanum lycopresicum, Synechococcussp. PCC 7942, Triticum aestivum,Ulvasp., symbiotic dinoflagellates ofStylophora pistillataandTurbinaria reniformis, Zea mays
• Not reactive in: No confirmed exceptions from predicted reactivity are currently known
• Immunogen: KLH-conjugated synthetic peptide derived from available plant, algal and cyanobacterial PsbA sequences, includingArabidopsis thalianaUniProt:A4QJR4, TAIR:AtCg00020,Oryza sativaP0C434,Populus albaQ14FH6,Physcomitrella patensQ6YXN7,Chlamydomonas reinhardtiiP07753,Synechocystissp.P14660and many others
• Clonality: Polyclonal
• Applications: Western blot (WB)
• Dilution: 1 :4000-1 : 8000, 5 µg of total protein, (WB)
• Purity: Purified, total IgY (chicken egg yolk immunoglobulin) in PBS pH 8. Contains 0.02 % sodium azide.
• Format: Liquid
• Molecular Weight: 38 | 28-30 kDa
• Precautions: The antibody is appropriate for detecting both, 24 kDa or the 10 kDa C-terminal fragments, whichever is generated under given treatment conditions.In our analysis we have seen both, ca. 24 kDa and ca. 10 kDa fragments from different samples, depending on treatments and isolation procedures.This antibody will also detect the phosphorylated form of D1as an alternate band to the main band on a high resolution gel.
• References & Citations: Rogowskiet al. (2023).Enzymatic kinetics of photosystem II with DCBQ as a substrate in extended Michaelis-Menten model. J Photochem Photobiol B. 2023 Oct:247:112780. doi: 10.1016/j.jphotobiol.2023.112780. Epub 2023 Aug 25.Vitaleet al.(2022) Manipulation of light quality is an effective tool to regulate photosynthetic capacity and fruit antioxidant properties of Solanum lycopersicum L. cv. 'Microtom' in a controlled environment. PeerJ. 2022;10:e13677. Published 2022 Jul 1. doi:10.7717/peerj.13677Toubianaet al. (2020). Correlation-based Network Analysis Combined With Machine Learning Techniques Highlight the Role of the GABA Shunt in Brachypodium Sylvaticum Freezing Tolerance. Sci Rep , 10 (1), 4489Sicoraet al. (2019). Regulation of PSII function in Cyanothece sp. ATCC 51142 during a light-dark cycle. Photosynth Res. 2019 Mar;139(1-3):461-473. doi: 10.1007/s11120-018-0598-5,Sevillaet al. (2019). Regulation by FurC in Anabaena links the oxidative stress response to photosynthetic metabolism. Plant Cell Physiol. 2019 May 21. pii: pcz094. doi: 10.1093/pcp/pcz094.Figlioliet al. (2019). Overall plant responses to Cd and Pb metal stress in maize: Growth pattern, ultrastructure, and photosynthetic activity. Environ Sci Pollut Res Int. 2019 Jan;26(2):1781-1790. doi: 10.1007/s11356-018-3743-y.Krupinskaet al. (2019). The nucleoid-associated protein WHIRLY1 is required for the coordinate assembly of plastid and nucleus-encoded proteins during chloroplast development. Planta. 2019 Jan 11. doi: 10.1007/s00425-018-03085-z.Sicoraet al. (2018). Regulation of PSII function in Cyanothece sp. ATCC 51142 during a light–dark cycle. Photosynth Res. 2018 Oct 24. doi: 10.1007/s11120-018-0598-5.Lentiniet al. (2018). Early responses to cadmium exposure in barley plants: effects on biometric and physiological parameters. Acta Physiol Plant (2018) 40: 178. doi: 10.1007/s11738-018-2752-2.GonzagaHeredia-Martinez et al. (2018). Chloroplast damage induced by the inhibition of fatty acid synthesis triggers autophagy in Chlamydomonas. Plant Physiol, Sept. 2018.Konget al. (2018) Interorganelle Communication: Peroxisomal MALATE DEHYDROGENASE2 Connects Lipid Catabolism to Photosynthesis through Redox Coupling in Chlamydomonas. Plant Cell. 2018 Aug;30(8):1824-1847. doi: 10.1105/tpc.18.00361.Dyet al. (2018). Galactoglycerolipid Lipase PGD1 Is Involved in Thylakoid Membrane Remodeling in Response to Adverse Environmental Conditions in Chlamydomonas. Plant Cell. 2018 Feb;30(2):447-465. doi: 10.1105/tpc.17.00446.Kimet al. (2018). The rice zebra3 (z3) mutation disrupts citrate distribution and produces transverse dark-green/green variegation in mature leaves. Rice (N Y). 2018 Jan 5;11(1):1. doi: 10.1186/s12284-017-0196-8.Yokonoet al. (2015). A megacomplex composed of both photosystem reaction centres in higher plants. Nat Commun. 2015 Mar 26;6:6675. doi: 10.1038/ncomms7675.Suet al. (2014). Exogenous progesterone alleviates heat and high light stress-induced inactivation of photosystem II in wheat by enhancing antioxidant defense and D1 protein stability. Plant Growth Regul DOI 0.1007/s10725-014-9920-1.Esparzaet al. (2013). Katanin Localization Requires Triplet Microtubules in Chlamydomonas reinhardtii. PLOS one.Hoogenboomet al. (2012). Effects of Light, Food Availability and Temperature Stress on the Function of Photosystem II and Photosystem I of Coral Symbionts. POLS one.Morashet al. (2007). Macromolecular dynamics of the photosynthetic system over a seasonal developmental progression in Spartina alterniflora. Canadian J. of Botany, 2007, 85(5): 476-483, 10.1139/B07-043.
• Storage Conditions: Store at 4°C; make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.