Anti-PsaB | PSI-B core subunit of photosystem I

Anti-PsaB | PSI-B core subunit of photosystem I

• Background: Photosystem I (PSI) of chloroplasts is a multisubunit membrane-protein complex that catalyzes the electron transfer from the reduced plastocyanin (or cytochrome c6) in the thylakoid lumen to the oxidized ferredoxin (or flavodoxin) in the chloroplast stroma. PsaB is a core protein of PSI complex. Synonymes: Photosystem I P700 chlorophyll a apoprotein A2, PSI-B
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Arabidopsis thaliana, Brassica napus, Brassica rapa, Bryopsis corticulans, Echinola crus-galli, Hordeum vulgare, Lolium perenne,Neochloris oleoabundans(chlorophyta), Mesostigma viride, Nicotiana tabacum, Phaeodactylum tricornutum, Pisum sativum, Setaria viridis, Solanum lycopersicum,Synechococcussp. PCC7942,Synechocystissp. PCC 6803,Triticum aestivum, Ulva prolifera, Zea mays
• Not reactive in: Chlamydomonas reinhardtii,dinoflagellate
• Immunogen: KLH-conjugated synthetic peptide derived from known PsaB sequences includingArabidopsis thalianaUniProt:P56767, TAIR:AtCg00340
• Clonality: Polyclonal
• Applications: Blue Native PAGE (BN-PAGE), Western blot (WB)
• Dilution: 1 : 1000 (BN-PAGE), (WB)
• Purity: Antigen affinity purified in PBS pH 7.4
• Format: Lyophilized
• Reconstitution: For reconstitution add 50 µl of sterile water
• Molecular Weight: 82.7 | 55-60 kDa
• Precautions: This product can be sold containing ProClin if requested
• References & Citations: Xieet al, (2024). LpY3IP1 Enhances the drought and salt tolerance of perennial ryegrass by protecting the photosynthetic apparatus. Scientia Horticulturae Volume 338, 1 December 2024, 113645.Tiwariet al. (2024). Differential FeS cluster photodamage plays a critical role in regulating excess electron flow through photosystem I. Nat Plants. 2024 Oct;10(10):1592-1603. doi: 10.1038/s41477-024-01780-2.Ermakovaet al. (2024). Chloroplast NADH dehydrogenase-like complex-mediated cyclic electron flow is the main electron transport route in C4 bundle sheath cells. New Phytol. 2024 Jul 22.doi: 10.1111/nph.19982.Grebeet al. (2024). Accounting for photosystem I photoinhibition sheds new light on seasonal acclimation strategies of boreal conifers. Journal of Experimental Botany, erae145Völkneret al. (2024). Evidence for partial functional overlap of KEA and MSL transport proteins in the chloroplast inner envelope of Arabidopsis thaliana. FEBS Lett. 2024 Aug;598(15):1877-1887. doi: 10.1002/1873-3468.14887.Jianget al. (2023). Toxic effects of lanthanum (III) on photosynthetic performance of rice seedlings: Combined chlorophyll fluorescence, chloroplast structure and thylakoid membrane protein assessment. Ecotoxicol Environ Saf. 2023 Nov 15:267:115627.doi: 10.1016/j.ecoenv.2023.115627.Seedpriming with NaCl helps to improve tissue tolerance, potassium retention ability of plants, and protects the photosynthetic ability in two different legumes, chickpea and lentil, under salt stress. Planta. 2023 May 8;257(6):111. doi: 10.1007/s00425-023-04150-y.Caoet al. (2023). An unexpected hydratase synthesizes the green light--absorbing pigment fucoxanthinKrynicka, et al. (2023) FtsH4 protease controls biogenesis of the PSII complex by dual regulation of high light-inducible proteins. Plant Commun. 2023;4(1):100502. doi:10.1016/j.xplc.2022.100505Lempiainenet al. (2022) Plants acclimate to Photosystem I photoinhibition by readjusting the photosynthetic machinery. Plant Cell Environ. 2022 Oct;45(10):2954-2971. doi: 10.1111/pce.14400. Epub 2022 Aug 16. PMID: 35916195.Zhaoet al. (2022) Native architecture and acclimation of photosynthetic membranes in a fast-growing cyanobacterium. Plant Physiol. 2022;190(3):1883-1895. doi:10.1093/plphys/kiac372Shuklaet al. (2020). A novel method produces native LHCII aggregates from the photosynthetic membrane revealing their role in non-photochemical quenching. J Biol Chem. 2020 Oct 20:jbc.RA120.016181. doi: 10.1074/jbc.RA120.016181. Epub ahead of print. PMID: 33082138.Griecoet al. (2020). Adjustment of photosynthetic activity to drought and fluctuating light in wheat. Plant Cell Environ. 2020 Mar 16. doi: 10.1111/pce.13756.Liuet al. (2020). Acid treatment combined with high light leads to increased removal efficiency of Ulva prolifera. Algal Research,Volume 45, January 2020, 101745Fredeet al. (2019). Light quality-induced changes of carotenoid composition in pak choi Brassica rapa ssp. chinensis. J Photochem Photobiol B. 2019 Apr;193:18-30. doi: 10.1016/j.jphotobiol.2019.02.001.Lima-Meloet al. (2019). Consequences of photosystem-I damage and repair on photosynthesis and carbon use in Arabidopsis thaliana. Plant J. 2018 Nov 29. doi: 10.1111/tpj.14177.Koochaket al. (2018). The structural and functional domains of plant thylakoid membranes. Plant J. 2018 Oct 12. doi: 10.1111/tpj.14127. (Blue Native PAGE)Gaoet al. (2018). Effect of green light on the amount and activity of NDH-1–PSI supercomplex in Synechocystis sp. strain PCC 6803. Photosynthetica (2018) 56: 316.Popovaet al. (2018). Differential temperature effects on dissipation of excess light energy and energy partitioning in lut2 mutant of Arabidopsis thaliana under photoinhibitory conditions. Photosynth Res. 2018 May 3. doi: 10.1007/s11120-018-0511-2.Rantalaand Tikkanen et al. (2018). Phosphorylation‐induced lateral rearrangements of thylakoid protein complexes upon light acclimation. Plant Direct Vol. 2, Issue 2.Wanget al. (2018). iTRAQ-based quantitative proteomics analysis of an immature high-oleic acid near-isogenic line of rapeseed. Molecular Breeding January 2018, 38:2.Kurkelaet al. (2017). Acclimation to High CO2 Requires the ω Subunit of the RNA Polymerase in Synechocystis. Plant Physiol. 2017 May;174(1):172-184. doi: 10.1104/pp.16.01953. Epub 2017 Mar 28.Schottleret al. (2017). The plastid-encoded PsaI subunit stabilizes photosystem I during leaf senescence in tobacco. J Exp Bot. 2017 Feb 1;68(5):1137-1155. doi: 10.1093/jxb/erx009.Giovanardiet al. (2017). Higher packing of thylakoid complexes ensures a preserved Photosystem II activity in mixotrophic Neochloris oleoabundans. Algal Research, Volume 25, July 2017, Pages 322-332.Jusovicet al. (2017). Photosynthetic Responses of a Wheat Mutant (Rht-B1c) with Altered DELLA Proteins to Salt Stress. Journal of Plant Growth Regulation, pp1-12.Georget al. (2017). Acclimation of Oxygenic Photosynthesis to Iron Starvation Is Controlled by the sRNA IsaR1. Curr Biol. 2017 May 22;27(10):1425-1436.e7. doi: 10.1016/j.cub.2017.04.010. (Synechocystis 6803 substrain PCC-M)Tyuerevaet al. (2017). The absence of chlorophyll b affects lateral mobility of photosynthetic complexes and lipids in grana membranes of Arabidopsis and barley chlorina mutants. Photosynth Res. 2017 Apr 5. doi: 10.1007/s11120-017-0376-9. (Hordeum vulgare, western blot)Gandiniet al. (2017). The transporter SynPAM71 is located in the plasma membrane and thylakoids, and mediates manganese tolerance in Synechocystis PCC6803. New Phytol. 2017 Mar 20. doi: 10.1111/nph.14526.Nathet al. (2016). A Nitrogen-Fixing Subunit Essential for Accumulating 4Fe-4S-Containing Photosystem I Core Proteins. Plant Physiol. 2016 Dec;172(4):2459-2470. Epub 2016 Oct 26.Fristedtet al. (2015). The thylakoid membrane protein CGL160 supports CF1CF0 ATP synthase accumulation in Arabidopsis thaliana. PLoS One. 2015 Apr 2;10(4):e0121658. doi: 10.1371/journal.pone.0121658.Suorsaet al. (2015). Light acclimation involves dynamic re-organisation of the pigment-protein megacomplexes in non-appressed thylakoid domains. Plant J. 2015 Aug 29. doi: 10.1111/tpj.13004.Griecoet al. (2015). Light-harvesting II antenna trimers connect energetically the entire photosynthetic machinery - including both photosystems II and I. Biochim Biophys Acta. 2015 Jun-Jul;1847(6-7):607-19. doi: 10.1016/j.bbabio.2015.03.004. Epub 2015 Apr 3.Subramanyamet al. (2014). Structural and functional changes of PSI-LHCI supercomplexes of Chlamydomonas reinhardtii cells grown under high salt conditions. Planta. 2010 Mar;231(4):913-22.Qinet al. (2014). Isolation and characterization of a PSI-LHCI super-complex and its sub-complexes from a siphonaceous marine green alga, Bryopsis Corticulans. Photosynth Res. 2014 Sep 12.Mustilaet al. (2014). The bacterial-type [4Fe–4S] ferredoxin 7 has a regulatory function under photooxidative stress conditions in the cyanobacterium Synechocystis sp. PCC 6803. BBA- Bioenergetics, April 2014.
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.