Anti-PsaC | PSI-C core subunit of photosystem I

Anti-PsaC | PSI-C core subunit of photosystem I

• Background: PsaCis a conserved, chloroplast-encoded, Fe-S binding protein of approximately 10 kDa, present in all known Photosystem I complexes. It is located on the stromal side of the thylacoid membranes. PsaC coordinates the Fe–S clusters FA and FB through two cysteine-rich domains.
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii, Cyanophora paradoxa, Dactylis glomeRata, Emiliania huxleyi, Euglena gracilis, Gonyaulax polyedra, Horderum vulgare, Lolium perenne, Marchantia polymorpha, Mesembryanthemum sp., Spinacia oleracea, Flaveria sp., Heterosigma akashiwo,Micromonas pusilla, Phaeodactylum tricornutum, Porphyra sp., symbiotic dinoflagellates of Stylophora pistillata and Turbinaria reniformis;  Synechococcussp.PCC7002, sp. PCC 7942, Synechocystis sp.PCC 6803, Thalassiosira pseudonana, Thalassiosira punctigera, Trichodesmium erythraeum, Triticum aestivum
• Not reactive in: No confirmed exceptions from predicted reactivity are currently known
• Immunogen: KLH-conjugated synthetic peptide conserved in all known PsaC proteins includingArabidopsis thalianaUniprot:P62090TAIR:AtCg01060,Hordeum vulgareUniProt:P69416,Oryza sativaUniProt:P0C360,Chlamydomonas reinhardtiiUniProt:Q00914,Synechococcus elongatusUniProt:Q31QV2
• Clonality: Polyclonal
• Applications: Western blot (WB)
• Dilution: 1 : 5000 (WB)
• Purity: Serum
• Format: Lyophilized
• Reconstitution: For reconstitution add 50 µl of sterile water
• Molecular Weight: 9 kDa
• Precautions: In some species minor cross reactions with some larger proteins are seen. These may contain related iron-sulfur binding motifs. Therefore size verification of the reacting band is required. Due to the small size of the protein, care should be taken to differentiate between chemiluminescent signal from PsaC and non-specific signals from chlotophylls or lipids if pigment is retained near the bottom of the blot.For the most optimal results use:thylakoid membranes or PSI particles,  solubilized in a SDS sample buffer (final concentrations: 63 mM Tris HCl, 10% glycerol, 2% SDS, 0.0025% bromophenol blue) with 2.5% beta-mercaptoethanol at 85C for 2 minutes. The samples were spun softly, then the supernatant loaded.This product can be sold containing ProClin if requested.
• References & Citations: Xieet al, (2024). LpY3IP1 Enhances the drought and salt tolerance of perennial ryegrass by protecting the photosynthetic apparatus. Scientia Horticulturae Volume 338, 1 December 2024, 113645.Hani and Krieger-Liszkay(2024). Manganese deficiency alters photosynthetic electron transport in Marchantia polymorpha. Elsevier Plant Physiology and Biochemistry Available online 16 August 2024, 109042.Frangedakiset al. (2024). MYB-related transcription factors control chloroplast biogenesis. Cell: DOI:https://doi.org/10.1016/j.cell.2024.06.039.Wuet al (2023) Disruption of LEAF LESION MIMIC 4 affects ABA synthesis and ROS accumulation in riceCano-Ramirezet al. (2023) Low-temperature and circadian signals are integrated by the sigma factor SIG5. Nat Plants. 2023 Apr;9(4):661-672. doi: 10.1038/s41477-023-01377-1. Epub 2023 Mar 30.Torrado, Connabeer, Rottig, et al. 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Structural and functional analyses of photosystem II in the marine diatom Phaeodactylum tricornutum. Proc Natl Acad Sci U S A. 2019 Aug 27;116(35):17316-17322. doi: 10.1073/pnas.1906726116.Zavrelet al. (2019). Quantitative insights into the cyanobacterial cell economy. Elife. 2019 Feb 4;8. pii: e42508. doi: 10.7554/eLife.42508.Zhanget al. (2019). Proteomic responses to ocean acidification of the marine diazotroph Trichodesmium under iron-replete and iron-limited conditions. Photosynth Res. 2019 May 10. doi: 10.1007/s11120-019-00643-8.Lupetteet al. (2019). The architecture of lipid droplets in the diatom Phaeodactylum tricornutum. Algal Research Volume 38, March 2019, 101415.Lima-Meloet al. (2019). Consequences of photosystem-I damage and repair on photosynthesis and carbon use in Arabidopsis thaliana. Plant J. 2018 Nov 29. doi: 10.1111/tpj.14177.Steinbecket al. (2018). Structure of a PSI-LHCI-cyt b6f supercomplex in Chlamydomonas reinhardtii promoting cyclic electron flow under anaerobic conditions. Proc Natl Acad Sci U S A. 2018 Oct 9;115(41):10517-10522. doi: 10.1073/pnas.1809973115.GonzagaHeredia-Martinez et al. (2018). Chloroplast damage induced by the inhibition of fatty acid synthesis triggers autophagy in Chlamydomonas. Plant Physiol, Sept. 2018.Liuet al. (2018). Effects of PSII Manganese-Stabilizing Protein Succinylation on Photosynthesis in the Model Cyanobacterium Synechococcus sp. PCC 7002. Plant Cell Physiol. 2018 Jul 1;59(7):1466-1482. doi: 10.1093/pcp/pcy080.Duet al. (2018). Galactoglycerolipid Lipase PGD1 Is Involved in Thylakoid Membrane Remodeling in Response to Adverse Environmental Conditions in Chlamydomonas. Plant Cell. 2018 Feb;30(2):447-465. doi: 10.1105/tpc.17.00446.Cantrelland Peers (2017). A mutant of Chlamydomonas without LHCSR maintains high rates of photosynthesis, but has reduced cell division rates in sinusoidal light conditions. PLoS One. 2017 Jun 23;12(6):e0179395. doi: 10.1371/journal.pone.0179395.Zanget al. (2017). Characterization of the sulfur-formation (suf) genes in Synechocystis sp. PCC 6803 under photoautotrophic and heterotrophic growth conditions. Planta. 2017 Jul 14. doi: 10.1007/s00425-017-2738-0.Huet al. (2017). The SUFBC2 D Complex is Required for the Biogenesis of All Major Classes of Plastid Fe-S Proteins. Plant J. 2017 Jan 19. doi: 10.1111/tpj.13483.Yang-ErChen et al. (2017). Responses of photosystem II and antioxidative systems to high light and high temperature co-stress in wheat. J. of Exp. Botany, Volume 135, March 2017, Pages 45–55.Liet al. (2016). A Hard Day's Night: Diatoms Continue Recycling Photosystem II in the Dark. Front. Mar. Sci., 08 November 2016Heinnickelet al. (2016). Tetratricopeptide repeat protein protects photosystem I from oxidative disruption during assembly. Proc Natl Acad Sci U S A. 2016 Mar 8;113(10):2774-9. doi: 10.1073/pnas.1524040113Rozpadeket al. (2015). The fungal endophyte Epichloë typhina improves photosynthesis efficiency of its host orchard grass (Dactylis glomerata). Planta. 2015 Jun 10.Subramanyamet al. (2014). Structural and functional changes of PSI-LHCI supercomplexes of Chlamydomonas reinhardtii cells grown under high salt conditions. Planta. 2010 Mar;231(4):913-22.Danget al. (2014). Combined Increases in Mitochondrial Cooperation and Oxygen Photoreduction Compensate for Deficiency in Cyclic Electron Flow in Chlamydomonas reinhardtii. Plant Cell. 2014 Jul 2. pii: tpc.114.126375.
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.